Yellow head virus (YHV) is an invertebrate nidovirus which causes a severe mortality in cultured Penaeus monodon. The mortality may be prevented by prior treatment of shrimps with YHV-protease dsRNA. Whether the YHV infected shrimp might be cured by the dsRNA remains to be investigated. P. monodon injected with 10(-6) YHV showed a high virus replication and mortality within 2 days. Injection of...

The population of Vibrios in diseased Penaeus monodon collected from culture ponds situated at Uran, Maharashtra (west coast of India) was studied. All animals collected were associated with more than one Vibrio species. Bacterial identification based on morphological and biochemical characteristics showed that four groups of Vibrios were present in shrimp hepatopancreas namely Vibrio parahaemo...

White spot syndrome virus (WSSV) is the causative agent of a severe disease of cultivated shrimp. Using purified WSSV virions, VP53A encoded by open reading frame wssv067 was identified as a structural protein by SDS-PAGE and proteomics. Immunoelectron microscopy with a gold-labeled secondary antibody revealed that VP53A was distributed on the viral envelope. In order to further explore the lin...

A cDNA microarray composed of 2,028 different ESTs from two shrimp species, Penaeus monodon and Masupenaeus japonicus, was employed to identify yellow head virus (YHV)-responsive genes in hemocytes of P. monodon. A total of 105 differentially expressed genes were identified and grouped into five different clusters according to their expression patterns. One of these clusters, which comprised fi...

Here we used both microsatellites and mtCR (mitochondrial DNA control region) sequences as genetic markers to examine the genetic diversity and population structure of Penaeus monodon shrimp from six Indonesian regions. The microsatellite data showed that shrimp from the Indian and the Pacific Ocean were genetically distinct from each other. It has been reported previously that P. monodon mtCR ...

The predominant carotenoid in prawn tissues is astaxanthin (Axn) and its role in pigmentation has been well studied. However, the effects of dietary Axn on other prawn physiological performance measures are uncertain and dietary carotenoid uptake and tissue deposition are poorly understood. This study fed juvenile prawns (Penaeus monodon) diets that contained 0, 25, 50 or 100 mg kg-1 Axn for 6 ...

We propose a novel method for direct cloning of foreign genes into baculoviruses which avoids the use of bacterial transfer vectors. The foreign gene to be inserted is derived by PCR using appropriate primers each of which contains an additional 50 nt of baculovirus sequence for homologous recombination between the PCR-derived DNA and the baculovirus DNA, thus accomplishing insertion of the for...

objective: influenza virus a (h1n1) is an important subtype of the influenza respiratory viruses, which has important worldwide implications. hemagglutinin (ha), an important viral antigen, is responsible for binding to human cell receptors leading to an onset of the disease process. considering the critical role of viral attachment, this study focuses on the extraction and cloning of ha and it...

Baculovirus infection of Spodoptera frugiperda cells is a system of choice to produce a range of recombinant proteins, vaccines and, potentially, gene therapy vectors. While baculovirus genomes are well characterized, the genome of S. frugiperda is not sequenced and the virus-host molecular interplay is sparsely known. Herein, we describe the application of stable isotope labeling by amino acid...