1. The proportion of active (dephosphorylated) pyruvate dehydrogenase in perfused rat heart was decreased by alloxan-diabetes or by perfusion with media containing acetate, n-octanoate or palmitate. The total activity, of the dehydrogenase was unchanged. 2. Pyruvate (5 or 25mm) or dichloroacetate (1m) increased the proption of active (dephosphorylated) pyruvate debydrogenase in perfused rat hea...

The oxidation of formaldehyde by rat liver mitochondria in the presence of 50 mM phosphate was enhanced 2-fold by exogenous NAD+. Absolute requirement of NAD+ for formaldehyde oxidation was demonstrated by depleting the mitochondria of their NAD+ content (4.6 nmol/mg of protein), followed by reincorporation of the NAD+ into the depleted mitochondria. Aldehyde (formaldehyde) dehydrogenase activi...

mitochondrial space was not completely reduced. This indicates some mitochondrial oxidative activity when alveolar Po, was in the 0.5-1.0 mrn Hg range. These studies have demonstrated substrate oxidation and respiratory control with isolated lung mitochondria. Measurements of surface fluorescence and tissue redox couples show intracellular reduction of pyridine nucleotides in response to oxidat...

This study aimed to evaluate the impact of a vapor freezing protocol on antioxidant enzyme activity (superoxide dismutase (SOD) and glutathione reductase (GR)), sperm with active mitochondria, DNA damage, spermatic parameters. Twenty-six semen samples from men undergoing infertility investigation were cryopreserved in liquid nitrogen (LN) vapors plunged into LN, (method A) without B) commercial...