The present research consisted of producing 3 types of powdered concentrates: the dairy, the egg and the dairy-egg (produced from a blend of raw liquid milk and liquid eggs). The dairy-egg powder was produced in order to facilitate formulation of dry food mixes. Powders were vacuumpackaged and packaged in air atmosphere and stored for 24 months. Changes in contents of selected oxysterols (deter...

Arsenic toxicity is a global health problem affecting many millions of people. The main source of exposure is drinking water contaminated by natural geological sources. Current risk assessment is based on the recognized carcinogenicity of arsenic, but neurotoxic risks have been overlooked. In 1955, an outbreak of arsenic poisoning occurred among Japanese infants, with more than 100 deaths. The ...

A simple, rapid and sensitive immunogold chromatographic strip test based on a monoclonal antibody was developed for the detection of melamine (MEL) residues in raw milk, milk products and animal feed. The limit of detection was estimated to be 0.05 μg/mL in raw milk, since the detection test line on the strip test completely disappeared at this concentration. The limit of detection was 2 μg/mL...

Milk protein concentrate (MPC) manufacture utilizes ultrafiltration to produce partially or completely delactosed high protein dairy ingredients. A variety of products are available in the liquid or dried form and range in protein content on a dry basis from 56 to 85%. The protein in MPC is a mixture of native casein and whey protein at a concentration of 80 and 20% of the total protein, respec...

A casein assay has been developed with aggregation induced emission character of BSPOTPE, which is a derivative of tetraphenylethene (TPE). The method exhibits a wide dynamic range of detection for casein from 10 μg ml(-1) to 5000 μg ml(-1), and a good linear range from 20 μg ml(-1) to 1250 μg ml(-1). With sample-pretreatment procedures, we can detect the casein in skimmed and full milk powder,...

Method Specific antibodies were raised in a Brown Norway (BN) rat model of food allergy. BN rats were dosed either (1) intraperitoneally (i.p.) with the purified native cow’s milk allergens with or without the use of Al(HO)3 as adjuvant or (2) orally with skimmed milk powder without the use of adjuvant. The specific IgG1 and IgE antibody responses were analysed against native and denatured alle...

Melamine can be lethal at high concentrations and has been used illegally to increase apparent protein content in food products including infant formula and milk powder. Milk tainted with melamine has caused death and illness in infants. Two years ago, the Codex Commission adopted a maximum melamine level of 1 mg/kg for powdered infant formula and of 2.5 mg/kg for other foods and animal feed. T...

A simple, precise, accurate, and validated reverse-phase HPLC method was developed for the determination of melamine in milk (pasteurized and UHT milk) and dairy products (powdered infant formula, fruit yogurt, soft cheese, and milk powder). Following extraction with acetonitrile:water (50:50, vol/vol), samples were purified by filter (0.45 μm), separated on a Nucleosil C8 column (4.6 mm × 250 ...

Three batches of yogurts were made with soy protein isolate (SPI) supplemented with 2% (S2), 4% (S4) or 6% (S6) of skim milk powder (SMP). The fourth batch (control; S0) was prepared from SPI without SMP supplementation. Lactobacillus delbrueckii ssp. bulgaricus ATCC 11842 (Lb 11842) and Streptococcus thermophilus ST 1342 (ST 1342) were used as the starter culture. Biotransformation of the inac...