Thrombin-induced clotting times were inversely proportional to fibrinogen concentrations within the range of 98 to 2900 nmol/L; these reciprocal velocity measurements had units of seconds per clot. By analogy with classical enzyme kinetics, we defined rectangular hyperbolic parameters where Km(clot) ranged from 0.14 to 0.56 mumol/L and kclot from 0.020 to 0.075 clot per second. Specificity rati...

BACKGROUND Rotational thromboelastometry (ROTEM®) is a point-of-care test for coagulation, enabling physicians to make a swift decision. The aim of this investigation was to establish reference intervals of thromboelastometric evaluation for coagulation in pediatric patients with congenital heart diseases (CHD). MATERIAL AND METHODS As baseline data, 3 assays of ROTEM® (INTEM, EXTEM, and FIBTEM...

The ratio of the milk-clotting activity (MCA) and proteolytic (PA) was compared for enzyme preparations (MEP) based on recombinant chymosin, chymosin animal origin microbial origin. MCA value measured in international units (IMCU), PA determined by Anson method substrate from hemoglobin at 30 °C рН 5.3. It found that chymosins had highest level MCA/ PA: group with < ~1000 includes MEPs Chy-m...

This study aimed to evaluate the milk clotting capacity (MCA), and caseinolytic activity (CA) of crude coagulant from stomachs adult rabbits (RC), their influence on composition, proteolysis, texture type Prato cheese. We tested two ways for salting abomasum three levels pH times enzyme activation. The presence enzymes in RC was assayed by SDS-PAGE. effects milk’s temperature RC’s water-holding...