Many new classes of Polymerase Chain Reaction (PCR) -based genetic markers have been developed over the last decade. Of these, microsatellite markers are widely regarded as one of the most useful identifi ed so far1,2,3. The signifi cance of microsatellite markers derives from their abundance in the genome, single locus nature, simplicity of assay, high levels of allelic diversity, mendelian in...

Microsatellites are composed of short tandem direct repeats; deletions or duplications of those repeats through the process of replication slippage result in microsatellite instability relative to other genomic loci. Variation in repeat number occurs so frequently that microsatellites can be used for genotyping and forensic analysis. However, an accurate assessment of the rates of change can be...

The MICdb is a comprehensive relational database of perfect microsatellites extracted from completely sequenced and annotated genomes of bacteria and archaea. The current version MICdb3.0 is an updated and revised version of MICdb2.0. As compared with the previous version MICdb2.0, the current release is significantly improved in terms of much larger coverage of genomes, improved presentation o...

MOTIVATION Simple sequence repeats or microsatellites have been found abundantly in many genomes. However, the significance of distribution preference has not been completely understood. Completion of the Arabidopsis genome sequencing allows us to better understand and characterize microsatellites. RESULTS Microsatellite distribution was more abundant in 5'-flanking regions of genes compared ...

Previously, we found that the genotype of 42 out of 198 mouse microsatellite loci, which are distributed among all chromosomes except the Y chromosome, changed from monomorphism to polymorphism (CMP) in a genetically modified inbred mouse strain. In this study, we further examined whether CMP also relates to the homologous recombination in gene knockout (KO) mouse strains. The same 42 microsate...

UNLABELLED PREMISE OF THE STUDY The current study aimed at developing nuclear microsatellite markers for the relict tree species Zelkova carpinifolia, which is threatened in its natural range in the South Caucasus. • METHODS AND RESULTS Pyrosequencing of an enriched microsatellite library on the Roche FLX platform using the 454 Titanium kit produced 86,058 sequence reads, most of which con...

UNLABELLED PREMISE OF THE STUDY Microsatellite loci for the native prairie perennial Echinacea angustifolia were developed and evaluated for future use in population structure and paternity studies. • METHODS AND RESULTS A total of 50 trinucleotide microsatellite regions were identified though an enrichment protocol that prescreens for microsatellite repeats before ligating into a vector. ...

We isolated 96 dinucleotide repeats with five or more tandemly repeated units from a subgenomic Drosophila subobscura library. The mean repeat unit length of microsatellite clones in D. subobscura is 15, higher than that observed in other Drosophila species. Population variation was assayed in 32-40 chromosomes from Barcelona, Spain, using 18 randomly chosen microsatellite loci. Positive correl...

PREMISE OF THE STUDY We designed and tested microsatellite markers for the North American native species Silene stellata (Caryophyllaceae) to investigate its population genetic structure and identify selection on floral design through male reproductive success. METHODS AND RESULTS A total of 153 candidate microsatellite loci were isolated based on next-generation sequencing. We identified 18 ...

UNLABELLED PREMISE OF THE STUDY Microsatellite markers from the genome of Diplarche multiflora were developed and characterized to investigate its genetic diversity and population structure. • METHODS AND RESULTS Twenty-seven microsatellite loci were isolated from the genome of D. multiflora using the Fast Isolation by AFLP of Sequences COntaining repeats (FIASCO) protocol. Of these marker...