نتایج جستجو برای: microplate

تعداد نتایج: 2640  

Journal: :Journal of immunological methods 1986
A D Smith J E Wilson

A modified ELISA procedure is described which permits selective detection of monoclonal antibodies reactive with the native antigen. The essence of the method is the use of immobilization methods that do not result in denaturation of the antigen during adsorption to the microtiter plates. This was accomplished by using polyclonal antibodies, adsorbed to the wells of the microtiter plate, as the...

Journal: :Antimicrobial agents and chemotherapy 1986
R C Cooksey B G Metchock C Thornsberry

We modified the phosphocellulose binding assay for aminoglycoside-modifying enzymes (AMEs) by use of microdilution plates and a multichannel micropipette. Batteries of aminoglycoside substrates for screening organisms for the presence of AMEs as well as for subclassifying enzymes were prepared and stored in microdilution plates. When tested in parallel with the conventional tube reaction assay,...

2004
Xiaopeng Zhao Eihab M Abdel-Rahman Ali H Nayfeh

We present a reduced-order model for electrically actuated microplate-based MEMS. The model accounts for the electric force nonlinearity and the mid-plane stretching of the plate. The linear undamped vibration modes are found numerically using the hierarchical finite-element method. These mode shapes are used in a Galerkin approximation to reduce the partialdifferential equations of motion and ...

2015
Paul Priyesh Vijayakumar Peter M. Muriana Jürg Bähler

Lactic acid bacteria (LAB) have historically been used in food fermentations to preserve foods and are generally-recognized-as-safe (GRAS) by the FDA for use as food ingredients. In addition to lactic acid; some strains also produce bacteriocins that have been proposed for use as food preservatives. In this study we examined the inhibition of Listeria monocytogenes 39-2 by neutralized and non-n...

Journal: :Antimicrobial agents and chemotherapy 2003
Chartchai Changsen Scott G Franzblau Prasit Palittapongarnpim

The green fluorescent protein (GFP) gene offers many advantages as a viability reporter for high-throughput antimicrobial drug screening. However, screening for antituberculosis compounds by using GFP driven by the heat shock promoter, hsp60, has been of limited utility due to the low signal-to-noise ratio. Therefore, an alternative promoter was evaluated for its enhanced fluorescence during mi...

Journal: :The Review of scientific instruments 2015
Chris Polin Nathan Wardlow Harold McQuaid Philip Orr Balder Villagomez-Bernabe Catarina Figueira Grace Alexander Shajeth Srigengan Emilie Brun Manon Gilles Cécile Sicard-Roselli Fred J Currell

Here is detailed a novel and low-cost experimental method for high-throughput automated fluid sample irradiation. The sample is delivered via syringe pump to a nozzle, where it is expressed in the form of a hanging droplet into the path of a beam of ionising radiation. The dose delivery is controlled by an upstream lead shutter, which allows the beam to reach the droplet for a user defined peri...

2015
Nabil E. Boutagy George W. Rogers Emily S. Pyne Mostafa M. Ali Matthew W. Hulver Madlyn I. Frisard

Skeletal muscle mitochondria play a specific role in many disease pathologies. As such, the measurement of oxygen consumption as an indicator of mitochondrial function in this tissue has become more prevalent. Although many technologies and assays exist that measure mitochondrial respiratory pathways in a variety of cells, tissue and species, there is currently a void in the literature in regar...

Journal: :Applied and environmental microbiology 2004
Mokhlasur Rahman Inger Kühn Motiur Rahman Barbro Olsson-Liljequist Roland Möllby

We describe the ScanMIC method, a colorimetric MIC method for susceptibility testing of gram-negative fermentative bacteria. The method is a slight modification of the National Committee for Clinical Laboratory Standards (NCCLS) recommended broth microdilution method that uses a redox indicator 2,3,5-triphenyltetrazolium chloride (TTC) to enhance the estimate of bacterial growth inhibition in a...

Journal: :Polish journal of microbiology 2012
Adel Almogren Zahid Shakoor Mustafa Hussein Adam Mohammad Osman Gadelrab Hassan Abdulaziz Musa

Reliability of the Widal tube agglutination test has been the subject of many controversies over the years. This study was performed to assess the effect of certain modifications on the performance of Widal test in a novel microplate assay. Sera from 37 patients (21 males; 16 females) (mean age 28 +/- 7 years) were tested in the Immunology Unit at King Khalid University Hospital, Riyadh. Among ...

M. Akhlaghi, M. S. Fereidouni, P. Akbary,

This study was conducted to examine in vitro antibacterialpotential from seed methanol extract of Peganum harmala (L) against some fish pathogenic bacteria including Lactococcus garvieae, Aeromonas hydrophila, Yersinia ruckeri and Pseuodomonas putida isolated from diseased rainbow trout (Oncorhynchus mykiss). The antibacterial activity of extracts was evaluated using disc diffusion assay, m...

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