cDNA cloning of a monoterpene synthase from Perilla frutescens whose steam-distilled oil contains 92.9% perillaketone, was performed by the PCR method using primers designed based on limonene synthase. The full-length nucleotide sequence of this cDNA consisted of 1978 bp including a 1827-bp translational region encoding a deduced protein of 608 amino acids, which was similar to that of limonene...

A methodology based on dispersive micro solid-phase extraction (DMSPE) using multiwalled carbon nanotubes (MWCNTs) as solid sorbent prior to measurement with energy-dispersive X-ray fluorescence (EDXRF) spectrometry is proposed. The procedure consists of chelating of nickel, cobalt, copper, and lead ions with ammonium pyrrolidinedithiocarbamate (APDC) and posterior adsorption of metal chelates ...

ELECTROSPINNING APPLIED TO THE DEVELOPMENT OF ADSORBENT NANOFIBERS AIMING AT IMPROVING SOLID PHASE (MICRO)EXTRACTION TECHNIQUES. The limitations and challenges associated with the complex analyte-matrixinterfering interactions make development of new sorbent materials an attractive line in several research areas, ranging from synthesis characterization to application these materials. Among diff...

This chapter focuses on three widely used techniques for extraction of semivolatile organics from liquids: liquid–liquid extraction (LLE), solid-phase extraction (SPE), and solid-phase microextraction (SPME). Other techniques may be useful in selected circumstances, but these three techniques have become the extraction methods of choice for research and commercial analytical laboratories. A fou...

dispersive liquid–liquid micro–extraction (dllme) technique was successfully used as a sample preparation method for the determination of clonazepam in pharmaceutical preparations and water samples. in this method, a suitable mixture of methanol (disperser solvent) and chloroform (extraction solvent) was injected rapidly into a conical test tube that contained an aqueous solution of clonazepam....

the growth rate of stem cells in mobius cellready 3l bioreactor has been studied using comsol multi-physics simulation software in order to find out the best operational parameters for secure cell growth. the bioreactor geometry was defined according to the emd millipore issued information while turbulent and unsteady state solid-liquid 2-phase fluid flow equations were used for modelling the b...

2 Introduction An important part of any analytical process is sample preparation, which involves isolation and preconcentration of various analytes of interest.1 Liquid–liquid extraction (LLE) is one of the oldest pretreatment procedures and because of its simplicity and low cost is commonly used. However, conventional LLE requires large volumes of high-purity solvents, which are often expensiv...