A practically simple top-down process for the exfoliation of graphene (GN) and few-layer graphene (FLG) from graphite is described. We have discovered that a biocompatible amphiphilic pyrene-based hexahistidine peptide is able to exfoliate, functionalize, and dissolve few layer graphene flakes in pure water under exceptionally mild, sustainable and virtually innocuous low intensity cavitation c...

Many proteins that accumulate in the form of insoluble aggregates when they are overproduced in Escherichia coli can be rendered soluble by fusing them to E. coli maltose binding protein (MBP), and this will often enable them to fold in to their biologically active conformations. Yet, although it is an excellent solubility enhancer, MBP is not a particularly good affinity tag for protein purifi...

"For the respect his works have commanded of musicians, and the popularity they have enjoyed among wider audiences, he is probably the most admired composer in the history of Western music." With this appraisal the New Grove Dictionary of Music and Musicians, published in 1980, begins its magisterial article on Beethoven. More than a decade later one might not apply this statement to the Teuton...

II Acknowledgements Praise is to Allah and gratitude is given where it is due most to Allah. So with genuine humility, I acknowledge the aid of Allah. My deepest gratitude and sincerest appreciation to Prof. Dr. Mohamed Hashem Abdel-Aziz, for his continuous encouragement and enlightening advice and helpful advices, that helped me throughout this work. I feel greatly indebted to Prof. Dr. Taymoo...

A surface plasmon resonance imaging-based Ni(2+)-iminodiacetic acid-coated gold chip system was developed to enable specific detection of a hexahistidine-tagged recombinant protein in crude extracts or in column chromatography fractions. This system is especially advantageous for high-throughput analysis of multiple proteins.

Fluorescent probes are essential for the exploration of protein function, detection of molecular interactions, and conformational changes. The nitrilotriacetic acid derivatives of different chromophores were successfully used for site-selective noncovalent fluorescence labeling of histidine-tagged proteins. All of them, however, suffer from the same drawback--loss of the fluorescence upon bindi...

Affinity tags have become powerful tools from basic biological research to structural and functional proteomics. They were widely used to facilitate the purification and detection of proteins of interest, as well as the separation of protein complexes. Here, we mainly discuss the benefits and drawbacks of several affinity or epitope tags frequently used, including hexahistidine tag, FLAG tag, S...

This paper grew out of a lecture presented at the 54th Session of the International Statistical Institute in Berlin, August 13 20, 2003, Schneeweiss (2003). It intends not only to outline the eventful life of Abraham Wald (1902 1950) in Austria and in the United States but also to present his extensive scientific work. In particular, the two main subjects, where he earned most of his fame, are ...

2015 2 Acknowledgement In the name of Allah, the Most Merciful, the Most Beneficient Firstly, I would like to thank the Almighty Allah for granting me the opportunity to come to New Zealand and undertake this research work. I would especially like to thank my husband for being there by my side and providing constant support and encouragement all through. I would like to thank my supervisor, Dr....