A common missense mutation in the methylenetetrahydrofolate reductase (MTHFR) gene, a C to T substitution at nucleotide 677, is responsible for reduced MTHFR activity and associated with modestly increased plasma homocysteine concentrations. Since underlying maternal vascular disease increases the risk of pre-eclampsia, we had the working hypothesis that pre-eclampsia patients would have an inc...

In Burkina Faso, the levels of plasma homocysteine (Hcy) are lower and the methionine loading tests suggest a more effective Hcy metabolism [1,2]. We found a relevant difference in the allele frequencies of C677T: T 7.7% in the young and 3.3% in old subjects respectively (see Table 1). Frequencies of C677T genotypes in old and young individuals were in Hardy Weinberg equilibrium (HWE). The diff...

BACKGROUND Hypermocysteinemia has been substantiated as a risk factor for occlusive vascular disease. A common mutation (nucleotide 677 C-->T) has been described recently in the 5,10-methylenetetrahydrofolate reductase (MTHFR) gene, which results in a valine for alanine substitution, a thermolabile enzyme, and a tendency to elevate plasma homocysteine levels and which has been proposed to contr...

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Recurrent pregnancy loss (RPL) defined as of two or more pregnancies is one the reasons why couples visit an ART clinic. 2-5% RPL cases show abnormal parental karyotype. Folic acid essential B vitamin involved in processes fundamental importance for cell division and embryo development. deficiency can have effect on many oocyte development, acquisition endometrial receptivity, implantation also...

The homogenate was subjected to centrifugation at 15 OOOg,, for IOmin and the residue was then washed with buffer A and re-centrifuged. The combined supernatant fractions were centrifuged at 23000g,,. for 60min and then stirred for 20min with DEAE-cellulose ( IOOml settled volume) that had been equilibrated in buffer A. The slurry was then poured into a column (lOx4cm) and washed with buffer A ...