Research was conducted to study the effects of biphasic anaerobic fermentation upon an organic fraction of municipal solid waste, and to observe the fate of heavy metals and pathogenic organisms in the acidogenic and methanogenic environments of the biphasic process. The high-solids (16 wt % solids) substrate was packed in a bench-scale, anaerobic, solid-state fermenter with amounts of lead nit...

OBJECTIVE The effects of lactic acid bacteria (LAB) and cellulase enzyme on fermentation quality, microorganism population, chemical composition and in vitro gas production of sorghum silages were studied. METHODS Commercial inoculant Lactobacillus plantarum Chikuso 1 (CH), local selected strain Lactobacillus casei (L. casei) TH 14 and Acremonium cellulase (AC) were used as additives in sorgh...

Nisin, a bacteriocin, is a potential alternative to antibiotics to modulate rumen fermentation. However, little is known about its impacts on rumen microbes. This study evaluated the effects of nisin (1 and 5 μM) on in vitro rumen fermentation characteristics, microbiota, and select groups of rumen microbes in comparison with monensin (5 μM), one of the most commonly used ionophores in ruminant...

The present study was carried out to evaluate the effect of methanolic extracts of three plants, mehandi (Lawsonia inermis), jaiphal (Myristica fragrans) and green chili (Capsicum annuum) on methanogenesis, rumen fermentation and fermentation kinetic parameters by in vitro gas production techniques. Single dose of each plant extract (1 ml / 30 ml buffered rumen fluid) and two sorghum fodder con...

The control of metabolite production is difficult in mixed culture fermentation. This is particularly related to hydrogen inhibition. In this work, hydrogenotrophic methanogens were selectively enriched to reduce the hydrogen partial pressure and to realize efficient acetate production in extreme-thermophilic (70°C) mixed culture fermentation. The continuous stirred tank reactor (CSTR) was stab...

Nitrate, 3-nitro-1-propionic acid (NPA) and 3-nitro-1-propanol (NPOH) can accumulate in forages and be poisonous to animals if consumed in high enough amounts. These chemicals are also recognized as potent anti-methanogenic compounds, but plants naturally containing these chemicals have been studied little in this regard. Presently, we found that nitrate-, NPA-, or NPOH-containing forages effec...

The caecal fermentation pattern, including methanogenesis, was studied in young rabbits using in vitro batch incubations. Six conventional litters of eight rabbits each were used. At the age of 22, 25, 28, 32, 36, 42 and 56 days, an animal was slaughtered from each litter and its caecal contents were used for in vitro batch incubations at 39 degrees C/24 h. The incubated samples were analysed f...

Phosphorus enrichment caused by runoff from agricultural areas has resulted in ecosystem-level changes in the northern Florida Everglades, including a loss of periphyton mats from nutrient-impacted areas. The potential for methanogenesis resulting from the anaerobic decomposition of cellulose and fermentation products, and the microorganisms responsible for these processes, were studied in mats...

Aim: The aim of the current study was to evaluate the effect of salinomycin in vitro on methanogenesis and rumen fermentation. Materials and Methods: Different levels of (0,10, 15 and 20 ppm) salinomycin were checked for their effect on in vitro methanogenesis and rumen fermentation on three wheat straw based diets i.e. low fiber diet (LFD, 40R:60C), medium fiber diet (MFD, 50R:50C) and high fi...

Ruminant animals play an important role in the food chain for evaluate cellulose and non-protein nitrogenous (NPN) compounds absorbed partially or not by other farm animals and humans. However, ruminant animals also bring some disadvantages. Methane, produced as a natural consequence of the ruminal digestion and it is a potential green house gas, is a problem, both ecologically and economically...