OBJECTIVES Acinetobacter baumannii is a significant pathogen in health care settings. In recent years, an increase in carbapenem resistance among A. baumannii due to Ambler class B metallo-beta-lactamases or class D OXA carbapenamases has been reported. In this study we detected the presence of OXA carbapenamases and coproduction of metallo-beta-lactamases (blaVIM and blaIMP ) by phenotypic and...

background and objectives : the rapid emergence and dissemination of carbapenemase-producing klebsiella pneumoniae strains and other members of the enterobacteriaceae poses a considerable threat to the care of hospitalized patients and to public health. the aim of this study was to determine the frequency of metallo-β-lactamases (mbl) and vim-1 gene in multidrug-resistant strains of k. pneumoni...

In this study, phenotypic and genotypic methods were used to detect metallo-β-lactamases, cephalosporinases and oxacillinases and to assess genetic diversity among 64 multiresistant Acinetobacter baumannii strains recovered from blood cultures in five different hospitals in Brazil from December 2008 to June 2009. High rates of resistance to imipenem (93.75%) and polymyxin B (39.06%) were observ...

BACKGROUND Infectious by Pseudomonas aeruginosa has spread worldwide and metallo-beta-lactamases (MBL) are being reported with increasing frequency. The aim of this study was to investigate the antibiotic susceptibility and distribution of blaVIM and blaIMP genes in P. aeruginosa isolates from Zanjan Province of Iran. METHODS A total of 70 P. aeruginosa isolates were identified from patients ...

The recent increase in gram-negative bacteria coproducing multiple classes of β-lactamases has made it difficult to accurately identify metallo-β-lactamase (MBL) producers. In the present study, six methods for detecting MBL producers were compared using 56 gram-negative bacterial isolates that produce various β-lactamases. Sodium mercaptoacetic acid (SMA) and EDTA were used as inhibitors for a...

Objective: In addition to assessing the phenotypic development of biofilms and beta-lactamases, in current study, Pseudomonas aeruginosa isolates from intensive care units (ICU) patients with prevalent drug resistance are being evaluated. Methods: Standard CLSI recommended protocols were utilized detect beta-lactamases such as extended-spectrum β-lactamases (ESBL), metallo beta-lactamase (MBL),...