Although plant responses to drought stress have been studied in detail in several plant species, including CAM plants, the occurrence of stress memory and possible mechanisms for its regulation are still very poorly understood. In an attempt to better understand the occurrence and possible mechanisms of regulation of stress memory in plants, we measured the concentrations of phytohormones in Ap...

The gene encoding a housekeeping form of phospboenolpyruvate carboxylase (EC 4.1.1.31), was isolated from a genomic library of total DNA of Mesembryanthemum crystallinum cloned into Lambda FIX (1) using a cDNA probe specific for the CAM (Crassulacean acid metabolism) form of the enzyme (2). This gene, designated Ppc2, is present as a single copy on the Af crystallinum genome. Unlike Ppcl, the e...

Mesembryanthemum crystallinum responds to salt stress by switching from C(3) photosynthesis to Crassulacean acid metabolism (CAM). During this transition the activity of phosphoenolpyruvate carboxylase (PEPCase) increases in soluble protein extracts from leaf tissue. We monitored CAM induction in plants irrigated with 0.5 molar NaCl for 5 days during the fourth, fifth, and sixth week after germ...

Cây Giọt băng (Mesembryanthemum crystallinum) là cây ưa mặn tuỳ nghi, có khả năng chịu cao, thậm chí sử dụng muối NaCl để thúc đẩy sinh trưởng. Đây một loài trồng giá trị với các mục đích khác nhau trong thực phẩm và dược phẩm. Nghiên cứu này đã đánh ảnh hưởng của môi trường dinh dưỡng cơ bản đến trưởng bằng kỹ thuật thuỷ canh hồi lưu dạng màng dưỡng, nhằm hướng việc thiết lập hệ thống sản xuất...

The plant-based-sea water culture medium is introduced to in vitro cultivation and in situ recovery of the microbiome of halophytes. The ice plant (Mesembryanthemum crystallinum) was used, in the form of juice and/or dehydrated plant powder packed in teabags, to supplement the natural sea water. The resulting culture medium enjoys the combinations of plant materials as rich source of nutrients ...

Ascochyta rabiei, the causal agent of Ascochyta blight on chickpea plants, secretes a cutinase in the culture filtrate when it is induced by cutin or hydroxylated fatty acids. This cutinase is the main esterase in the culture fluids. The enzyme was purified to homogeneity by three successive chromatographic steps. It showed an apparent molecular weight of 22 kD in SDS-PAGE and cleaved ester bon...

Strains of Ascochyta rabiei which are pathogenic to chickpea (Cicer arietinum L.) readily catabolized the main chickpea isoflavone biochanin A (5,7-dihydroxy-4'-methoxyisoflavone). 3'-Hydroxylation and O-demethylation reactions led to the isoflavones pratensein, genistein, and orobol, which were rapidly further degraded. Dihydrogenistein and p-hydroxyphenylacetic acid were also identified as ca...

Ascochyta blight caused by Ascochyta rabiei (Pass.) Labrousse is a major biotic constraint in production of chickpea. In the present investigation, all chickpea genotypes [E100Y (m), Gaurav, Pb-7 and L550] induced 100% callus on standard medium with greenish colour and fragile structure. These calli were used for in vitro screening against pathogen, A. rabiei culture filtrate at 0, 5, 10, 15 an...

Halophytes are salt-tolerant plant that grows naturally in saline areas where almost all conventional crops die due to NaCl toxicity. The common ice plant, Mesembryanthemum crystallinum L., an annual halophyte native South Africa, tolerates high salinity levels and accumulates a shoot at level. To check the availability of for desalinization soils, we cultured soils collected from 16 sites loca...