Monoclonal antibodies (mAbs) that react with soluble mercuric ions have been produced by injection of BALB/c mice with a hapten-carrier complex designed to maximize exposure of the metal to the immune system. Three hybridomas producing antibodies that reacted with bovine serum albumin (BSA)-glutathione-HgCl, but not with BSA-glutathione, were isolated from the spleen of a mouse given multiple i...

Three sets of experiments were carried out to determine if there is an intestinal secretory component in the fecal excretion of administered inorganic mercury. In the first set of experiments the disposition of a nontoxic 0.5-micromol/kg intravenous dose of inorganic mercury was evaluated in control rats and rats whose bile duct had been ligated. Data collected 24 h after the administration of ...

BACKGROUND Methicillin-resistant Staphylococcus aureus (MRSA) continues to be a major cause of serious infections in hospitals and in the community worldwide. In this study, MRSA isolated from patients in Kuwait hospitals were analyzed for resistance trends and the genetic location of their resistance determinants. METHODS Between April 1994 and December 2004, 5644 MRSA isolates obtained from...

目 的 作業現場での水銀への暴露 による神経系 の障害 は,産 業衛生上の問題 として取 り上げ られている1).水 銀 によ る神経系の障害発現 には神経細胞の脱落が関与 している ことが知 られている. PC12細 胞はラッ トの副腎よ り得 られた褐色細胞腫由 来の株化細胞で,神 経成長菌子(NGF)に 反応 して神 経突起の成長やカテコールア ミンの合成促進等の神経細 胞様分化を起こす ことが知 られ,神 経細胞 の分化機構の 研究等に多 く用 いられている細胞株 であ る2). 本研究 では, NGFに よって神経細 胞様 に分化 した PC12細 胞 を塩化第二水銀を添加 した培地中で培養 した ときに起 こる細胞死が, RNA合 成阻害剤,蛋 白合成阻 害剤もしくはエ ンドヌクレアーゼ阻害剤の添加で抑制で きるか否かを明 らかにする事 によ り,水 銀暴露 に よる P...

In embryological work using vital dyes it is highly desirable to be able to study the distribution of the dyes in fixed material, whether examined in toto or after embedding in paraffin and serial sectioning. However, both fixation and dehydration present problems for the preservation of colour in vitally stained cells. Some fixatives preserve some dyes but, so far as I am aware, none will pres...

The current study was aimed at isolating and identifying the halophilic and halotolerant bacteria which can produce mercuric reductase in Gavkhuni wetland in Iran. Moreover, tracking and sequencing merA gene and kinetic properties of mercuric reductase in the selected strain were performed in this study. Soil samples were taken from Gavkhuni wetland and cultured in nutrient agar medium...