Fluorescent nucleic acid detection in polymerase chain reaction (PCR) generally uses oligonucleotide probes labeled with covalently attached dyes. However, unlabeled oligonucleotides in the presence of saturating DNA dyes can also serve as hybridization probes. The DNA dye, LCGreen Plus, and a 3'-blocked unlabeled probe are added before amplification, and asymmetric PCR is performed at a 1:5 to...

S. T. Yang, M. Zhou, T. Jiang, X. X. Xue, P. N. Duan, W. J. Zhang: School of Metallurgy, Northeastern University, China Melting behavior is the precondition of liquid phase reaction, which is the foundation of sinter consolidation. Melting property measurement and sinter pot test were adopted to determine the melting temperature and strength of sinter with 13 % and 40 % vanadium-titanium ore ad...

The melting behavior of an Ag microwire mesh induced by Joule heating was numerically investigated and compared with that of the corresponding Ag nanowire mesh with the same structure but different geometrical and physical properties of the wire itself. According to the relationship of melting current and melting voltage during the melting process, a similar repetitive zigzag pattern in melting...

rate’ of cpx and opx. Isobaric melting experiments show INTRODUCTION a > b, but the abyssal peridotite data suggest b > a, i.e. I recently showed (Niu, 1997) that proportions of residual opx would contribute more than cpx to the melt during minerals in abyssal peridotites (e.g. Dick, 1989; Johnson melting beneath ocean ridges. As abyssal peridotites et al., 1990; Johnson & Dick, 1992) exhibit s...

Comment on " Vortex Liquid Crystal in Anisotropic Type II Superconductors " The suppression of superconductivity in type II superconductors is associated with the melting transition of the lattice of flux quanta [1, 2, 3]. Most melting phenomena in Nature are thermodynamic first-order phase transitions. An analytic description of melting is thus difficult since there is no good handle, such as ...

The melting behaviour of d-sucrose, d-glucose and d-fructose was studied. The melting peaks were determined with DSC and the start of decomposition was studied with TG at different rates of heating. In addition, melting points were determined with a melting point apparatus. The samples were identified as d-sucrose, alpha-d-glucopyranose and beta-d-fructopyranose by powder diffraction measuremen...

A recent significant achievement in trace element modeling is the development of the congruent (eutectic) dynamic melting (CDM) model where only melt is generated. However, melting reactions in the mantle and crust often produce not only melt but also minerals. By introducing melting reactions into the dynamic melting model, we present a series of equations for incongruent dynamic melting (IDM)...

Crystal engineering concept has been utilized to modify the physico-chemical parameters of a naturally occurring alkaloid, quinine sulphate, by exploring its H-bond interactions to generate different forms. Quinine sulphate is found to exist in four different crystal forms. The Forms I and II depict endo/exo events suggesting conversion of metastable low melting forms to higher melting and stab...

Melting of crystalline solids ~superlattices! of octadecanethiol and octanethiol protected silver clusters has been studied with x-ray powder diffraction ~XRD!, differential scanning calorimetry ~DSC!, and infrared ~IR! spectroscopy. These solids have been compared with the silver thiolate layered compounds in view of their similarity in alkyl chain packing and x-ray diffraction patterns. Super...

How does DNA melt in columnar aggregate relative to its melting in diluted solution? Is the melting temperature increased or decreased with the aggregate density? Have DNA-DNA interactions, predominantly of electrostatic nature, an effect on the character of the melting transition? In attempt to answer these questions, we have incorporated the theory of electrostatic interactions between DNA du...