The assay medium contained SO~M-KCI, ZOm~-Tricine, 0.83 mM-EDTA, 1 mMsuccinate, 5 m ~ N a ~ s O ~ , 10 % (w/v) sucrose, 50,u~-carbonyl cyanide p-trifluoromethoxyphenylhydrazone, final pH7.6, in a total volume of 2.0ml. Reaction was started by the addition of chromatophores to give 20,u~-bacteriochlorophyll in assay medium. Release of phosphate was measured colorimetrically after 3 min at 30°C i...

In this research, tunability of a commercial diode laser has extended to about more than ± 11 nm using a V-shaped external-cavity fabricated around the laser. Although under normal condition it can be tuned up to about ± 4 nm just by changing its temperature and injection current. Such modified diode laser has then used in a difference-frequency generation (DFG) experimental setup as pump sourc...

HeLa cells in culture synthesize metallothionein. To investigate the effects of glucocorticoids on metallothionein synthesis we adapted HeLa cells to growth in a defined medium lacking hydrocortisone. In this defined medium, containing 1.5 x 10(-6)M Zn2+, dexamethasone (10(-6)M) caused a five- to sixfold increase in the synthesis of metallothionein. Progesterone (10(-5)M), a known antagonist of...

A medium is described which selectively inhibits most bacteria from the normal flora of the conjunctiva, the lid-margin, and the upper respiratory tract but permits growth of Moraxella nonliquefaciens, M. liquefaciens, and the less fastidious strains of M. lacunata. The selective conditions are obtained with thiostrepton, tyrothricin, and sodium nalidixinate as inhibitors. Incubation under incr...

Until recently, genetic analysis of Mycobacterium tuberculosis, the causative agent of tuberculosis, was hindered by a lack of methods for gene disruptions and allelic exchange. Several groups have described different methods for disrupting genes marked with antibiotic resistance determinants in the slow-growing organisms Mycobacterium bovis bacillus Calmette-Guérin (BCG) and M. tuberculosis. I...

The laboratory diagnosis of tuberculosis usually relies on culture-based isolation of the causative Mycobacterium tuberculosis bacteria. We developed and evaluated the performance of MOD9, a new blood-free derivative of the MOD4 solid medium on which we previously reported for the isolation and culture of mycobacteria. First, inoculation of Lowenstein-Jensen medium with 21 M. tuberculosis isola...

Conditioned medium was collected from vascular smooth-muscle cells grown in culture to determine if these cells synthesize vasoactive substances. The medium caused a short-acting endothelium-independent constriction of rat aorta, followed by a prolonged, endothelium-dependent relaxation. This relaxation was mediated through the release of endothelium-derived relaxing factor (EDRF) as it was abo...

Conditioned medium was collected from vascular smooth-muscle cells grown in culture to determine if these cells synthesize vasoactive substances. The medium caused a short-acting endothelium-independent constriction of rat aorta, followed by a prolonged, endothelium-dependent relaxation. This relaxation was mediated through the release of endothelium-derived relaxing factor (edrf) as it was abo...

PURPOSE To evaluate the role of the radiometric BACTEC 460TB system and the conventional Lowenstein-Jensen (LJ) medium for isolation of M. tuberculosis from cerebrospinal fluid (CSF) samples of tuberculous meningitis (TBM) patients. METHODS CSF specimens (n=2325) from suspected TBM patients were processed for isolation of mycobacteria by inoculating BACTEC 12B medium and the LJ medium. The is...