We report the development of a combined strategy for high capacity, comprehensive enrichment of endogenous peptide from complex biological samples at natural pH condition. MCM-41 nanoparticles with highly ordered nanoscale pores (i.e. 4.8nm) and high-surface area (i.e. 751m(2)/g) were synthesized and modified with strong cation-exchange (SCX-MCM-41) and strong anion-exchange (SAX-MCM-41) groups...

The MCM proteins are a group of six proteins whose action is vital for DNA replication in eukaryotes. It has been suggested that they constitute the replicative helicase, with a subset of the proteins forming the catalytic helicase (MCM4,6,7) while the others have a loading or control function. In this paper we show that all six MCM proteins are present in equivalent amounts in soluble extracts...

BACKGROUND The aim of this study was to assess the metallothionein (MT), maintenance protein 2 (Mcm-2) and Ki-67 expressions in adrenocortical adenomas and carcinomas in comparison to normal tissue and evaluate the correlations between these markers of proliferation and between these markers and tumor diameter. MATERIALS AND METHODS The expression of MT, Mcm-2 and Ki-67 was assessed by immuno...

The hexameric MCM complex is the catalytic core of the replicative helicase in eukaryotic and archaeal cells. Here we describe the first in vivo analysis of archaeal MCM protein structure and function relationships using the genetically tractable haloarchaeon Haloferax volcanii as a model system. Hfx. volcanii encodes a single MCM protein that is part of the previously identified core group of ...

The minichromosome maintenance (MCM) complex is thought to function as the replicative helicase in archaea, separating the two strands of chromosomal DNA during replication. The catalytic activity resides within the C-terminal region of the MCM protein, while the N-terminal portion plays an important role in DNA binding and protein multimerization. An alignment of MCM homologues from several ar...

During late M and early G(1), MCM2-7 assembles and is loaded onto chromatin in the final step of prereplicative complex (pre-RC) formation. However, the regulation of MCM assembly remains poorly understood. Cyclin-dependent kinase (CDK)-dependent phosphorylation contributes to DNA replication by initially activating pre-RCs and subsequently inhibiting refiring of origins during S and M phases, ...

The recently proposed Minimal Complexity Machine (MCM) finds a hyperplane classifier by minimizing an exact bound on the Vapnik-Chervonenkis (VC) dimension. The VC dimension measures the capacity of a learning machine, and a smaller VC dimension leads to improved generalization. On many benchmark datasets, the MCM generalizes better than SVMs and uses far fewer support vectors than the number u...

We present a new technique for content based image retrieval using motif cooccurence matrix(MCM). MCM is derived using a motif transformed image. The whole image is divided into 2 2 pixel grids. Each grid is replaced with the scan motif which minimize the local gradient while traversing the 2 2 grid forming a motif transformed image. MCM is then de ned as a 3 dimensional matrix whose (i,j,k) en...

MCM: mucormycosis PAS: periodic acideSchiff INTRODUCTION Mucormycosis (MCM) is a fungal infection from members of the Mucorales order of fungi found almost exclusively in immunocompromised patients. The number of these infections seems to be increasing, presumably paralleling the increasing numbers of people with diminished immune surveillance. Primary cutaneous MCM in healthy patients is rare ...

This paper presents a new approach based on multicarrier modulation (MCM) with OFDM, to accomplish the goals of 4G. MCM [1] is a form of multicarrier system and a derivative of frequency-division multiplexing. MCM is a baseband process that uses parallel equal bandwidth sub channels to transmit information. Normally implemented with Fast Fourier transform (FFT) techniques, MCM's advantages incl...