BACKGROUND & OBJECTIVES Pathogenic bacteria often cause life threatening infections especially in immunocompromised individuals. Therefore, rapid and reliable species identification is essential for a successful treatment and disease management. We evaluated a rapid, proteomic based technique for identification of clinical bacterial isolates by protein profiling using matrix-assisted laser deso...

•Large-molecule decomposition products in SEIs are revealed with MALDI-TOF-MS •On-electrode chromatography is employed to assist MALDI measurements •The molecular SEI components characterized directly on the electrode surfaces •This approach can be applied a variety of battery systems The solid-electrolyte interphase (SEI) produced by electrolyte has critical impact performance. However, precis...

Multilocus sequence typing (MLST) is an important tool for the global surveillance of bacterial pathogens that is performed by comparing the sequences of designated housekeeping genes. We developed and tested a novel mass spectrometry-based method for MLST of Streptococcus pneumoniae. PCR amplicons were subjected to in vitro transcription and base-specific cleavage, followed by analysis of the ...

Background: Foodborne infections like Listeriosis cover several disorders and are a worldwide public health emergency. Listeria monocytogenes has been isolated from various foodstuffs, including milk fishes. MALDI-TOF mass spectrometry technique is known for its rapid accurate identification of bacterial organisms. Methods: In the current research, total 360 samples comprising raw (130), produc...

Over the past few decades there has been an increased interest in using various analytical techniques for detecting and identifying microorganisms. More recently there has been an explosion in the application of matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS) for bacterial characterization, and here we optimize this approach in order to generate repro...

The identification of pharmacologically promising compounds (lead compounds) from combinatorial libraries is frequently limited by the throughput of the analytical technique employed. Fourier transform mass spectrometry (FTMS) offers high sensitivity, mass accuracy (m/Deltam > 500 000), and sequencing capabilities. A rapid and efficient method for high-throughput analysis of single beads from p...

In this study we compare the capability of amplification fragment-length polymorphism (AFLP) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) to identify and subtype isolates of members of the Candida parapsilosis complex (C. parapsilosis, C. orthopsilosis, C. metapsilosis) and Lodderomyces elongisporus, which cannot be differentiated with biochemi...

The certification of a polystyrene standard reference material, SRM 2888, is described. The Mw of SRM 2888 was determined by light scattering to be 7.19x10 g/mol with a sample standard deviation of 0.14 x10 g/mol. A combined expanded uncertainty for this light scattering Mw determination, including systematic and random uncertainties, was estimated to be 0.57 x10 g/mol. Mn was determined by nuc...

Abstract In this study, matrix-assisted laser desorption ionisation time of flight mass spectrometry (MALDI-TOF MS) was used to identify bacteria from environmental matrices. The aim work determine the efficacy rapid technique and bacterial community agricultural samples. Environmental samples included collection irrigation waters manures, surface vegetables were also investigated. From food sa...

Characterising the protein signatures in tumours following vascular-targeted therapy will help determine both treatment response and resistance mechanisms. Here, mass spectrometry imaging and MS/MS with and without ion mobility separation have been used for this purpose in a mouse fibrosarcoma model following treatment with the tubulin-binding tumour vascular disrupting agent, combretastatin A-...