The aim of this study was to detect the presence carbapenem resistant Escherichia coli (E. coli) in cattle and pigs. Altogether, 100 samples were collected, 50 each from pigs including mastitic milk faecal healthy as well diarrhoeic slaughter specimens Bacteria isolated antibiotic sensitivity test carried out. Eight isolates E. obtained 15 phenotypic resistance patterns against carbapenems (ert...

The current study was planned to investigate the potential risk factors and their association with mastitis in Cholistani cattle. For this purpose milk samples were collected and examined for mastitis using California Mastitis Test. The results showed an overall 21.96% prevalence of subclinical mastitis. The analysis of variance did not show significant association of tail size, live body weigh...

In a national survey of US dairy operations, 1685 dairy operations reported 47,057 new dairy heifers (either births or acquisitions) and 4427 deaths (9.4%) of preweaned dairy heifer calves over a 3 month period. Stepwise logistic regression was used to identify management practices associated with high mortality among preweaned heifers in dairy operations where at least three dairy heifer calve...

The present work is a large epidemiological study aiming to detect the prevalence of subclinical mastitis and to investigate the major udder pathogens in Jalisco State, western Mexico. For this purpose, 2205 dairy cows, representing 33 Mexican dairy herds, were involved. Of 2205 cows, 752 mastitic animals were diagnosed and only 2,979 milk samples could be obtained for further investigation. Al...

The relationship between preinfection functions of blood neutrophils and outcome of experimental Escherichia coli mastitis was studied in 11 cows. Random migration, chemotaxis, phagocytosis, and chemiluminescence by neutrophils were determined in white blood cell suspensions, and in purified neutrophil suspensions. The course of E. coli mastitis (10(4) E. coli 0:157 in rear quarters) was monito...

In more than 30% of milk samples from clinical and subclinical bovine mastitis, bacteria fail to grow even after 48 h of conventional culture. The "no-growth" samples are problematic for mastitis laboratories, veterinarians, and dairy producers. This study provides the first investigation of the bacteriological etiology of such samples, using a real-time PCR-based commercial reagent kit. The as...

This study was conducted in certain area at Khartoum State (Eltebna, Falasteen, Shambat, Hilat Kuku, Elhalfaia, Elsamrab and The University of Khartoum farms) in winter season to determine the type of mastitis and to compare between the incidence of mastitis caused by Stapylococcus spps and Bacillus spp. The total number of dairy cows, which were examined in 34 investigated farms, amounted to 5...

staphylococci isolated from 98 mastitic cow’s milk samples were analyzed for the production of alpha,beta and delta-hemolysin on sheep, bovine and horse red blood cells and in vitro susceptibility to several antimicrobial agents. out of a total of 18 isolated strains, 100% demonstrated hemolytic activity either alone or in combined forms. among 8 isolates of s. aureus 3 isolates produced α/β an...