Atherosclerosis is an inflammatory disease, and the involvement of immune mechanisms in disease progression is increasingly recognized. Immunization with oxidized low density lipoprotein (LDL) decreases atherosclerosis in several animal models. To explore humoral and cellular immune reactions involved in this protection, we immunized apolipoprotein E knockout mice with either homologous plaque ...

Malondialdehyde (MDA) is an end-product of lipid peroxidation and a side product of thromboxane A(2) synthesis. Moreover, it is not only a frequently measured biomarker of oxidative stress, but its high reactivity and toxicity underline the fact that this molecule is more than "just" a biomarker. Additionally, MDA was proven to be a mutagenic substance. Having said this, it is evident that ther...

This experimental study was designed to determine effects of Nigella sativa oil (NSO) on endothelin-1 (ET-1) level and oxidative stress parameters, superoxide dismutase (SOD) and malondialdehyde (MDA) in a rat sepsis model. Twenty four adult Wistar albino rats were divided randomly into three groups: sham group (group 1), sepsis group (group 2), sepsis group pretreated with NSO (group 3). Serum...

Using high performance liquid chromatographic methods, both plasma and urine lipoperoxide concentrations were measured, as malondialdehyde (MDA), in 30 stable renal transplant patients receiving daily cyclosporine and/or azathioprine therapy. Their MDA concentrations were compared with previously reported reliable reference values using the same liquid chromatographic methods. Although their pl...

A reliable method for spectrophotometric determination of urinary malondialdehyde (MDA), according to the thiobarbituric acid (TBA) assay, is described. To account for matrix interference and differences in individual urine composition, standard addition procedure was applied. The method is adequately selective (LoQ = 0.09 μM in the presence of 0.1 M creatinine and 0.5 M urea) and reliable (wit...

Malondialdehyde (MDA) is a natural and widespread genotoxin. Given its potentially deleterious effects, it is of interest to establish the identities of the cell types containing this aldehyde. We used in situ chemical trapping with 2-thiobarbituric acid and mass spectrometry with a deuterated standard to characterize MDA pools in the vegetative phase in Arabidopsis thaliana. In leaves, MDA occ...

ABSTRACTDiazinon has a hepatotoxic effect since it is metabolized in the liver involving Kupffer cells (KCs) activation, increasing reactive oxygen species (ROS). The flavonoids contained shallot (Allium cepa L.) skin act as antioxidants neutralizing oxidative stress. This study aims to determine of ethanol extract (SSEE) on MDA level and activated KCs histopathology. total SSEE was measured us...

this study was conducted to assess the effect of ginger supplementation on malondialdehyde (mda) level, as an oxidative stress marker, and total antioxidant capacity (tac) in broiler chicks. two hundred male 1-day-old chicks were assigned randomly to four dietary treatments (basal diet as control group and basal diet containing 2.5, 5.0 and 7.5 g/kg ginger, respectively), with five replicates a...

Malondialdehyde (MDA) is an endogenous genotoxic product of enzymatic and oxygen radical-induced lipid peroxidation whose adducts are known to exist in DNA isolated from healthy human beings. To evaluate the mutagenic potential of MDA in human cells, we reacted MDA with pSP189 shuttle vector DNA and then transfected them into human fibroblasts for replication. MDA induced up to a 15-fold increa...

Submit Manuscript | http://medcraveonline.com Abbreviations: DM: Diabetes Mellitus; CVDs: Cardiovascular Diseases; PQQ: Pyrroloquinoline Quinone; LDH: Lactate Dehydrogenase; RR-value: Relative Risk Value; LPO: Lipid Peroxidation; LOOH: Lipid Hydroperoxide; HDL-C: High Density Lipoprotein; ROS: Reactive Oxygen Species; MDA: Malondialdehyde; STZ: Streptozotocin; PBS: Phosphate Buffered Saline; BS...