Phylogenetic relationships among heterobasidiomycetous yeasts, including anamorphic and teleomorphic taxa, have been compared from the sequence similarity of small (18S) and large (25S) subunit ribosomal RNA. Species examined were Cystofilobasidium capitatum, Filobasidiella neoformans, Fi/obasidium floriforme, Leucosporidium scottii, Malassezia furfur, M. pachydermatis, Phaffia rhodozyma, Rhodo...

Although the fungal microbiome in human skin mainly comprises lipophilic yeasts, including Malassezia species, these microorganisms can cause various dermatitis conditions, pityriasis versicolor, seborrheic dermatitis, folliculitis, and atopic depending on host condition. Both globosa restricta are major species implicated Malassezia-related dermatitis. However, pathogenicity of has not been re...

The genus Malassezia has been considered an emergent pathogen [4] and could be found in seborrheic dermatitis lesions as spherical yeast cell shapes and in pityriasis versicolor forming short hyphae with yeast cells [3]. Picrosirius is used in histology to stain collagen [5] and has been also used to stain Cryptoccoccus spp. and Paracoccidioides brasiliensis [1,2]. However, there are not studie...

A wide variety of bacteria and fungi are found on the human skin. Although some skin microorganisms produce antibacterial peptides that inhibit invasion by pathogens or promote the integrity of cutaneous defenses by eliciting host immune responses, the normal microbiome can also cause several skin diseases. Atopic dermatitis (AD) is a chronic disease that causes pruritus and involves cycles of ...

The objective of this study was to use nanohybrid Curcumin Cur-ZnO and Cur-MgO as well free curcumin detect, isolate, characterize, inhibit the development certain yeasts locally isolated from some spoilage foods. Yeasts responsible for spoiling were recovered in 21 different colonies cheese, cooked pickles, fig jam, apple dates, rice, canned chickpeas, dried figs. Nine yeast isolates chosen fu...

Malassezia spp. are lipid-dependent yeasts that have been related to skin mycobiota and dermatological systemic diseases. Study of lipid droplets (LDs) is relevant elucidate the unknown role these organelles in gain a broader overview metabolism Malassezia. Here, we standardized two protocols for analysis LDs M. pachydermatis globosa. The first describes co-staining confocal laser-scanning fluo...

background: malassezia are dimorphic, lipid-dependent yeasts, which are responsible for causing several cutaneous and sys­temic conditions. although cyclophilins (cyps) are highly conserved cytosolic proteins that catalyze the peptidyl-prolyl cis-trans isomerazation reaction before protein folding process, it has been suggestive of an allergen in a few numbers of fungi such as aspergillus fumig...

Fungal DNA was selectively amplified, and the ITS region sequenced, from fecal samples taken from 45 healthy human volunteers at one (21 volunteers) or two (24 volunteers) time points. Seventy-two operational taxonomic units, representing two phyla and ten classes of fungi, were recovered. Candida yeasts, notably Candida tropicalis (present in 51 samples), and yeasts in the Dipodascaceae (39 sa...

Reference methods for antifungal susceptibility testing of yeasts have been developed by the Clinical and Laboratory Standards Institute (CLSI) and the European Committee on Antibiotic Susceptibility Testing (EUCAST). These methods are intended to test the main pathogenic yeasts that cause invasive infections, namely Candida spp. and Cryptococcusneoformans, while testing other yeast species int...