An immunocapture reverse transcription polymerase chain reaction (IC-RT-PCR) method for a highly sensitive analysis of raspberry bushy dwarf virus (RBDV) in infected plants is described. In the method, preliminary purification of virus particles or viral RNA from the plant material is not necessary. Viruses are enriched during the assay by antibodies bound in the PCR microplate wells, followed ...

SUMOylation is an essential ubiquitin-like modification involved in important biological processes in eukaryotic cells. Identification of small ubiquitin-related modifier (SUMO)-conjugated residues in proteins is critical for understanding the role of SUMOylation but remains experimentally challenging. We have set up a powerful and high-throughput method combining quantitative proteomics and pe...

in order to study the effect of intensity and exposure duration magnetic field on wheat (triticum aestivum l.) seed germination an experiment was conducted in laboratory of ferdowsi university of mashhad, iran. the experiment was including 11 treatments as three level of magnetic field intensities (pretreatment of seeds with 50, 100 and 150 mt magnetic field) and three exposure durations (10, 2...