Helium displacement and nitrogen adsorption techniques were used to determine the density and porosity, respectively, of freeze-dried cell walls isolated from Bacillus megaterium KM and Saccharomyces cerevisiae. The densities were 1.302 and 1.180 g/cm3, respectively, suggesting noncrystalline solids. The porosities were extremely small, indicating that the cell walls had collapsed and become es...

The mixed system between p-sulfonatocalix[4]arene and tetradecyltrimethylammonium bromide forms unilamellar vesicles after sonication of the aqueous dispersion. Furthermore these vesicles can be stored, without use of lyoprotectants, by lyophilization and then rehydration without change in size or shape.

Abstract: Background: Paliperidone is indicated for the treatment of schizophrenia. It has an absolute oral bioavailability about 28% as it poorly soluble in water and also undergoes hepatic first-pass metabolism. Objectives: The purpose present study to improve solubility, vitro paliperidone by formulating nanostructured lipid carrier (NLC). Materials Methods: High shear homogenization followe...

The broadest clinical application of siRNA therapeutics will be facilitated by drug-loaded delivery systems that maintain stability and potency for long times under ambient conditions. In the present study, we seek to better understand the stability and effect of storage conditions on lipidoid nanoparticles (LNPs), which have been previously shown by our group and others to potently deliver RNA...

An overview of the state-of-the-art in the use of lyophilization for the pretreatment of samples and standards prior to their storage and/or preconcentration is presented. The different analytical applications of this process are dealt with according to the type of material (reagent, standard, samples) and matrix involved.

Traditional method of the routine subculturing by transfer of fungal cultures from staled to fresh media is not a very practical means of storing large numbers of fungal cultures. It is time-consuming, prone to contamination, and does not prevent genetic and physiological changes. At present, besides freeze-drying (lyophilization), cryopreservation seems to be the best preservation technique av...

BACKGROUND A large gap for the support of point-of-care testing is the availability of reagents to support quality control (QC) of diagnostic assays along the supply chain from the manufacturer to the end user. While reagents and systems exist to support QC of laboratory screening tests for glucose-6-phosphate dehydrogenase (G6PD) deficiency, they are not configured appropriately to support poi...

Tilmicosin (TLM) is an important antibiotic in veterinary medicine with low bioavailability and safety. This study aimed to formulate and evaluate physicochemical properties, storage stability after lyophilization, and antibacterial activity of three TLM-loaded lipid nanoparticles (TLM-LNPs) including solid lipid nanoparticles (SLNs), nanostructured lipid carriers (NLCs), and lipid-core nanocap...