Previously, we demonstrated that hypoxia (1% O2) enhances stemness markers and expands the cell numbers of cochlear stem/progenitor cells (SPCs). In this study, we further investigated the long-term effect of hypoxia on stemness and the bioenergetic status of cochlear spiral ganglion SPCs cultured at low oxygen tensions. Spiral ganglion SPCs were obtained from postnatal day 1 CBA/CaJ mouse pups...

background: spermatogonial stem cells are subpopulation of spermatogonial cells in testis tissue that support beginning and maintenance of spermatogenesis. ubiquitin carboxy-terminal hydrolase l1 (uchl1) could be a specific marker for identification of spermatogonial stem cells including spermatogonial sperm cells (sscs) in testis tissue and during the culture; therefore we undertook this study...

introduction: previous studies indicate that exercise influences cognitive function. nevertheless, considering that exercise in animal study can be voluntary, or forced, effects of exercise (specially forced exercise) on learning and memory abides as a matter of controversy. the present study aimed to investigate the effects of treadmill exercise on ltp in the dentate gyrus of rats. methods: th...

introduction: over the last decade, exposure to non-ionizing electromagnetic waves due to base station antenna has increased. this in vivo study was planned for evaluating the effects of whole-body exposure to 950 mhz field of gsm mobile phone system on rat dentate gyrus long-term potentiation. materials and methods: 24 naive male wistar rats (3 month old, 225|¡|25 g) were randomly divided in t...

sewage effluent is the carrier of huge amounts of n (0.09 to 0.16%), p (0.023 to 0.029 %), and k (0.11 to 0.18%) and as well as it is rich in organic carbon (3.27%). transfer of the toxic trace metal (pb) from the contaminated site can be seized out from the food chain through application of feso4.7h2o and caso4 in a larger area. the efficacy of the toxic metal immobilization through an applica...

The long-term ecological response to recurrent deforestation associated with shifting cultivation remains poorly investigated, especially in the dry tropics. We present a study of phosphorus (P) dynamics in the southern Yucatán, highlighting the possibility of abrupt shifts in biogeochemical cycling resulting from positive feedbacks between vegetation and its limiting resources. After three cul...

Extensive expansion of mesenchymal stem cells (MSCs) for cell-based therapies remains challenging since long-term cultivation and excessive passaging in two-dimensional conditions result in a loss of essential stem cell properties. Indeed, low survival rate of cells, alteration of surface marker profiles, and reduced differentiation capacity are observed after in vitro expansion and reduce ther...

It has previously been shown that the nucleotide sequences of the Agrobacterium rhizogenes rolC locus and the selective marker nptII developed mutations during the long-term cultivation of transgenic cell cultures of Panax ginseng. In the present report, we analyzed the nucleotide sequences of selected plant gene families in the 20-year-old P. ginseng 1c cell culture and in leaves of cultivated...

Nine encoded wine samples from a German long-term field trial on the comparison of different cultivation systems were examined with the image forming methods biocrystallization, capillary dynamolysis and circular chromatography. The images of the encoded samples were i. differentiated into three groups of images with similar form expression, ii. characterised as ‘fresh – aged’ based on a catalo...

Shifting cultivation is a traditional agricultural practice in most tropical regions of the world and has the potential to provide for human livelihoods while hosting substantial biodiversity. Little is known about the resilience of shifting cultivation to increasing agricultural demands on the landscape or to unexpected disturbances. To investigate these issues, we develop a simple social-ecol...