Here we report a novel self-assembly induced solubilization strategy with nanostructured ionic liquids as solvents. Highly ordered mesoscopic structures featuring the solute as a key component, such as liquid crystals, were formed via self-assembly in nanostructured long-chain carboxylate ionic liquids, resulting in extremely high solubilities for sparingly soluble drug molecules.

inoculation of phosphate-solubilizing microorganisms along with rock phosphate is known to enhance the available p from soil. the success of such solubilization is largely dependent on the ability of the inoculant strain to survive under adverse environmental conditions. in this context, liquid inoculants are gaining importance and are becoming popular with longer shelf-life. in the present inv...

This article reviews our current understanding of the uptake of fatty acids by the enterocytes of the intestine. The micellar solubilization of fatty acids by bile salts and the factors regulating that process are discussed. The mechanism of how micellar solubilization of fatty acids promotes the uptake of fatty acids by enterocytes and their relative importance is reviewed. Additionally, discu...

It has been previously shown that trypsin treatment of rat liver plasma membranes causes the solubilization of a guanylate cyclase of Mr = 140,000 (Lacombe, M. L., Haguenauer-Tsapis, R., Stengel, D., Ben Salah, A., and Hanoune, J. (1980) FEBS Lett. 116, 79-84). In this study, we observed that addition of Mn-GTP during this step greatly protected the enzyme from proteolytic degradation. This eff...

1. Mitochondria were isolated from developing endosperm of Ricinus communis and were fractionated into outer membrane and inner membrane. The relative purity of the two membrane fractions was determined by marker enzymes. The fractions were also examined by negative-stain electron microscopy. 2. Membrane fractions were sequentially extracted in the following way. (a) Suspension in 0.5M-potassiu...

Detergents are indispensable in the isolation of integral membrane proteins from biological membranes to study their intrinsic structural and functional properties. Solubilization involves a number of intermediary states that can be studied by a variety of physicochemical and kinetic methods; it usually starts by destabilization of the lipid component of the membranes, a process that is accompa...

The serotonin 1A receptor is an important member of the G-protein coupled receptor family, and is involved in a variety of cognitive, behavioral, and developmental functions. None of the subtypes of G-protein coupled serotonin receptors have yet been purified to homogeneity from natural sources. We report here, for the first time, the solubilization of human serotonin(1A) receptors stably expre...

Membrane proteins comprise one of the major challenges in structural biology. Membrane protein complexes pose particular difficulties because they not only require careful solubilization, but additionally the structural, as well as functional integrity, of the entire complex needs to be maintained. Electron crystallography of two-dimensional (2D) crystals is an important emerging approach in th...

CDP-1,2-diacyl-sn-glycerol (CDP-diacylglycerol):myo-inositol phosphatidyltransferase (EC 2.7.8.11, phosphatidylinositol synthase) catalyzes the final step in the de novo synthesis of phosphatidylinositol in the endoplasmic reticulum fraction of germinating soybeans (Glycine max L. var Cutler 71). A variety of solubilization agents were examined for their ability to release phosphatidylinositol ...

The efficacy of strains of Pseudomonas fluorescens, Bacillus megaterium and Azospirillum spp. in in vitro solubilization of Ca3PO4 was studied. Pseudomonas fluorescens and Bacillus megaterium strains were the most powerful phosphate solubilizers on Pikovskaya (PVK) plates and liquid medium. Azospirillum lipoferum strains showed weak zones of solubilization on the PVK plates. Phosphate solubiliz...