Antigen tests for infectious diseases are inexpensive and easy-to-use, but the limit of detection (LOD) is generally higher than that PCR tests, which considered gold standard. In present study, we combined a sandwich enzyme-linked immunosorbent assay (ELISA) with thionicotinamide-adenine dinucleotide (thio-NAD) cycling to improve LOD antigen coronavirus disease 2019 (COVID-19). For recombinant...

BackgroundThrombotic thrombocytopenic purpura (TTP) is a rare but potentially fatal disorder caused by ADAMTS13 (a disintegrin and metalloproteinase with thrombospondin type 1 motif, member 13) deficiency. Prompt identification/exclusion of TTP can thus be facilitated rapid testing. The most commonly utilized (enzyme?linked immunosorbent assay [ELISA]–based) takes several hours to perform so do...

To ascertain the prevalence of BHV-1 antibodies among cattle population, Virus neutralization test (VNT) and indirect Enzyme linked immunosorbent assay (ELISA) were employed for the detection of BHV-1 antibodies. Evaluation of these two serological tests was made to find out the rapid, economic and sensitive diagnostic test for detection of BHV-1 infection. A total of 255 serum samples were col...

This study aims to determine of Chloramphenicol test for Vannamei shrimp conducted ELISA (Enzyme-Linked Immunosorbent Assay) method with 1 treatment. is done by adding the Abenzyme conjugate, and ends addition substrate reaction stop buffer. Each treatment was performed three times. The value (CAP) (Litopenaeus vannamei) which tested at 4 weeks ranged from 0.021 0.153 ppb. result showed in stil...

fast enzyme linked immunosorbent assay (fast-elisa) was compared with the standard elisa for the diagnosis of human hydatidosis. seventy serum samples including 30 from hydatidosis patients (surgically confirmed), healthy control individuals not infected with any parasitic diseases (n=/20) and from others with different parasitic infections including, toxocariosis (n=5), fasciolosis (n=5), tric...

Plant diseases caused by viruses are the most menace to sustainable agriculture, leading numerous billion dollars in losses annually. Proper and accurate detection of plant is always key developing appropriate solutions manage economic them. Advances technology have simplified tools available detect diagnose at a time when control measures should be used. Currently, nucleic acid serological bas...