Background: Norepinephrine plays a trophic role in the control of cell replication and differentiation in target cells that express adrenergic receptors. Methods: In this study, we have tested the influence of infraphysiological, physiological and supraphysiological concentrations (0.0001 nM, 1 nM, 10000 nM) of human norepinephrine on the proliferation of breast cancer cells (human breast adeno...

fms-like tyrosine kinase-3 is a receptor tyrosine kinase expressed by immature hematopoietic cells and is important for the normal development of stem cells and the immune system. mutations of fms-like tyrosine kinase-3 have been detected in about 30% of patients with acute myelogenous leukemia and a small number of patients with acute lymphoblastic leukemia. the fms-like tyrosine kinase-3 muta...

background: p19 cells are mouse embryonic carcinoma cells which contain pluripotent ability, like stem cells, to differentiate into different cell lines. there are several properties for this cell line that make it a valuable cell model for study of developmental stages. methods: at the first step, pts2-egfp coding sequence which was cloned in pucd2.hygro vector was used for transfection in to ...

The possibility to reprogram somatic human cells will greatly and deeply change genetic approach and allow the development of new tools to study genetics diseases. Indeed, our ability to study human genetic diseases suffers from the lack of valid in vitro models. The latter should (i) be originating from human primary cells, (ii) be able to self-renew for a long time and (iii) be able to differ...

Objective(s): Design and construction of biocompatible and biodegradable scaffolds are among the main goals of tissue engineering. Recently, use of nano-hydroxyapatite as a bioactive bioceramic agent with high similarity to the mineral phase of the human bone tissue, in combination with biodegradable polymers and implant coatings has attracted the attention of researchers in the field of biomat...

background: bone marrow stromal stem cells (bmsc) are appropriate source of multipotent stem cells that are ideally suited for use in various cell-based therapies. it can be differentiated into neuronal-like cells under appropriate conditions. this study examined the effectiveness of co-stimulation of creatine and retinoic acid in increasing the differentiation of bmsc into gabaergic neuron-lik...

background: adult stem cells (asc) are undifferentiated cells found throughout the body. these cells are promising tools for cell replacement therapy in neurodegenerative disease. adipose tissue is the most abundant and accessible source of asc. this study was conducted to evaluate effect of selegiline on differentiation of adipose-derived stem cells (adsc) into functional neuron-like cells (nl...

Background: Human ESCs-MSCs open a new insight into future cell therapy applications, due to their unique characteristics, including immunomodulatory features, proliferation, and differentiation. Methods: Herein, hESCs-MSCs were characterized by IF technique with CD105 and FIBRONECTIN as markers and FIBRONECTIN, VIMENTIN, CD10, CD105, and CD14 genes using RT-PCR technique. FACS was performed fo...

background: to evaluate the effect of exendine-4 (ex-4), a glucagon-like peptide 1 (glp-1) receptor agonist, on the differentiation of insulin-secreting cells (ipcs) from rat adipose-derived mesenchymal stem cells(admscs). materials and methods: in this experimental study, admscs were isolated from rat adipose tissue and exposed to induction media with or without ex-4. after induction, the exis...

objective(s): nowadays much effort is being invested in order to diagnose the mechanisms involved in neural differentiation. by clarifying this, making desired neural cells in vitro and applying them into diverse neurological disorders suffered from neural cell malfunctions could be a feasible choice. thus, the present study assessed the capability of fetal brain extract (fbe) to induce rat bon...