To investigate the seroprevalence of leptospiral infection in sheep in Ahvaz, southwestern Iran, bloodsamples were taken from 181 female sheep. Sera were stored at -20°C until use. They were initially screenedat serum dilution of 1:100 against six live antigens of Leptospira interrogans serovars pomona, canicola,hardjo, ballum, icterohaemorrhagiae, grippotyphosa using the microscopic agglutinat...