Cytoprotective ability of polysaccharides isolated from different edible mushrooms was investigated on the 7-ketocholesterol-induced damaged cell line. Polysaccharide extracts from six different edible mushrooms-Flammulina velutipes, Peurotus ostreatus, Lentinus edodes, Agrocybe aegerita, Agaricus blazei, and Cordyceps militaris- were prepared by hot water extraction and alcohol precipitation. ...

A laccase with a molecular mass of 67 kDa and inhibitory activity toward HIV-1 reverse transcriptase (IC50 = 7.5 microM) was isolated from fresh fruiting bodies of the Lentinus edodes (Shiitake mushroom). Its characteristics were compared with those of laccases from cultured mushroom mycelia reported earlier. The laccase was unadsorbed on DEAE-cellulose, Affi-gel blue gel and CM-cellulose, but ...

The scavenging activity of polysaccharides from Lycium barbarum, Lentinus edodes and Ganoderma Lucidum Karst to DPPH free radicals was investigated. It was found that among the three polysaccharides, Lycium barbarum polysaccharides (LBP) exhibits the best scavenging activity. Polysaccharide loaded collagen membranes were prepared by mixing LBP with collagen, starch, glycerol, sodium carboxymeth...

Four crude water soluble polysaccharides, CABP, CAAP, CFVP and CLDP, were isolated from common edible mushrooms, including Agaricus bisporus, Auricularia auricula, Flammulina velutipes and Lentinus edodes, and their chemical characteristics and antioxidant properties were determined. Fourier Transform-infrared analysis showed that the four crude polysaccharides were all composed of β-glycoside ...

Using C3H/He mice, the antitumor effect of lipopolysaccharide (LPS) alone and in combination with Lentinan extracted from Lentinus edodes was studied. The influence of LPS on cellular immunity and the antitumor effect of the tumor necrosis factor (TNF) were also examined. LPS, which was administered into mice with tumor, induced hemorrhagic necrosis of the tumor within 48 h, demonstrating a hig...

Reversing the function of immune suppressor cells may improve the efficacy of cancer therapy. Here, we have isolated a novel polysaccharide MPSSS (577.2 Kd) from Lentinus edodes and examined its effects on differentiation and function of myeloid-derived suppressor cells (MDSCs). MPSSS is composed of glucose (75.0%), galactose (11.7%), mannose (7.8%), and xylose (0.4%). In vivo, it inhibits the ...

DNA isolation from some fungal organisms is difficult because they have cell walls or capsules that are relatively unsusceptible to lysis. Beginning with a yeast Saccharomyces cerevisiae genomic DNA isolation method, we developed a 30-min DNA isolation protocol for filamentous fungi by combining cell wall digestion with cell disruption by glass beads. High-quality DNA was isolated with good yie...

In this paper, the theory and construction methods of four models are presented for predicting the vegetable market price, which are BP neural network model, the neural network model based on genetic algorithm, RBF neural network model and an integrated prediction model based on the three models above. The four models are used to predict the Lentinus edodes price for Beijing Xinfadi wholesale m...

Mevcut çalışma ile yenilebilir bir kültür mantarı olan Lentinus edodes misellerinin (LEM) vejetatif gelişmesi üzerine farklı ligno-selülozik maddelerin etkileri incelenmiştir. Bu amaçla; Mısır Unu (MU), Pirinç (PU) ve Buğday Ununun (BU), %20, %15, %10 %5’lik konsantrasyonları MU, PU, BU karışımı malt ekstrakt kullanıldı. Misel Gelişim Süresi (MGS) besi yerlerinin etkisi gösterilmiştir. Tohumluk...