background and objectives: loop-mediated isothermal amplification is a novel nucleic acid amplification assay providing as a simple diagnostic tool for rapid identification of microbial diseases in developing countries. in this study, a lamp assay was established for yersinia enterocolitica , a leading cause of acute enterocolitis in young children. materials and methods: lamp assay was establi...

BACKGROUND Cystic echinococcosis (CE) or hydatidosis, caused by the larval stage of Echinococcus granulosus (EG)-complex, is a neglected parasitic disease of public health importance. The disease is endemic in many African and Mediterranean countries including the Sudan. The objective of the present study was to develop and evaluate a real-time loop-mediated isothermal amplification (LAMP) assa...

The sensitivity of LAMP, PCR and microscopy to detect Theileria spp. and Trypanosoma congolense in field-derived bovine blood samples from Tanzania was evaluated and compared. No parasites were detected by microscopy. Furthermore, no bovine Theileria spp. were detected by LAMP and PCR from all the 24 samples collected from Arusha. Four and one out of 24 samples were positive for Theileria congo...

Pigment disorders1,3 Hypopigmentation or depigmentation. Lesions demonstrate increased sharpness of borders under Wood lamp examination and fluoresce bright blue-white owing to the increased amount of dermal collagen illuminated. This results from decreased or absent intervening melanin. Vitiligo: patches, varying in location and extent. Tuberous sclerosis: patches; characteristic shapes are la...

James Thomson envisaged a lamp which would be turned on for 1 minute, off for 1/2 minute, on for 1/4 minute, etc. ad infinitum. He asked whether the lamp would be on or off at the end of 2 minutes. Use of “internal set theory” (a version of nonstandard analysis), developed by Edward Nelson, shows Thomson’s lamp is chimerical; its copy within set theory yields a contradiction. The demonstration ...

BACKGROUND Buruli ulcer (BU) caused by Mycobacterium ulcerans (M. ulcerans) has emerged as an important public health problem in several rural communities in sub-Saharan Africa. Early diagnosis and prompt treatment are important in preventing disfiguring complications associated with late stages of the disease progression. Presently there is no simple and rapid test that is appropriate for earl...

Here we describe a method for the detection of Clostridium difficile from stool using a novel low-complexity and rapid extraction process called Heat Elution (HE). The HE method is two-step and takes just 10 minutes, no specialist instruments are required and there is minimal hands-on time. A test method using HE was developed in conjunction with Loop-mediated Isothermal Amplification (LAMP) co...