Bacteria are known to adapt to environmental changes such as temperature fluctuations. It was found that temperature affects the lysis-lysogeny decision of lambda such that at body temperature (37 degrees C) the phage can select between the lytic and lysogenic pathways, while at ambient temperature (20 degrees C) the lytic pathway is blocked. This temperature-dependent discriminatory developmen...

SYSTEM of genetic transduction has been discovered in the sexually fertile A K-12 strain of Escherichia coli. This transduction is mediated by lambda, a temperate phage for which K-12 is normally lysogenic. The distinctive features of the lambda-K-12 system include the following: (1) The transductions are limited to a cluster of genes for galactose fermentation. The GaZ loci are closely linked ...

INTRODUCTION ...................................................... 52 INHIBITION OF PHAGE REPLICATION BY LAMBDA LYSOGENS ..... ...... 53 Inhibition of T-Even rI Mutants ............................................ 63 Inhibition of Ti and T5 ............................6......................... 5 INHIBMON BY OTHER LYSOGENIZED PHAGE 65 P2and P2-Like-Induced Abortive Infections...66 P22-Induced ...

Taylor, M. W. (Stanford University, Stanford, Calif.), and C. Yanofsky. Chromosomal relocation of prophage-associated bacterial genes. J. Bacteriol. 91:1469-1476. 1966.-Two distinguishable colony types, rough-edged and smooth-edged, were observed when tryptophan auxotrophs of Escherichia coli were transformed to tryptophan independence with DNA from the hybrid nondefective transducing phage i(l...

Lambda coli phage is not inactivated by chymotrypsin, trypsin, or ficin. T(2) phage is slowly inactivated by high concentrations of (alpha-, beta-, gamma-, or Delta-chymotrypsin, but not by trypsin or ficin. P(1) phage is slowly inactivated by alpha-, beta-, or gamma-chymotrypsin, or ficin, more rapidly by Delta-chymotrypsin, and much more rapidly by trypsin. Crystalline egg albumin, crystallin...

Highly purified HindIII restriction fragments of Xenopus laevis 5S DNA and of Psammechinus miliaris histone DNA have been covalently inserted into a derivative of phage lambda. This phage, genetically constructed by Murray et al. (1), contains only a single target for HindIII in the cI gene. Viable hybrid molecules were detected as clear plaque-forming phage after transfection of E. coli, the v...