In recent publications, Wallenfels (1) and Aronson (2) have reported that new oligosaccharides are produced during the hydrolysis of lactose by enzyme preparations from bacteria and yeasts. The enzymatic synthesis of oligosaccharides from lactose has also been observed by Pazur (3) and by Roberts and McFarren (4). It has been suggested (l-3) that the new compounds are the result of transgalacto...

Fowler, Audree V. (University of California, Los Angeles), and Irving Zabin. Effects of dimethylsulfoxide on the lactose operon in Escherichia coli. J. Bacteriol. 92:353-357. 1966.-Dimethylsulfoxide (DMSO) at a concentration of 5% (v/v) in the culture medium inhibits the growth of Escherichia coli to only a slight extent, and does not affect the differential rate of synthesis of beta-galactosid...

Buffer type, pH and ionic strength, as well as the fraction of polyvinylpyrrolidone were optimized for efficient extraction of b-galactosidase from almond seeds. The enzyme was purified up to electrophoretic homogeneity employing (NH4)2SO4 (15–60 %) fractionation, size exclusion and ion-exchange chromatography. Molecular mass of b-galactosidase as estimated by gel filtration and SDS-PAGE was ap...

Epilactose is a functional sugar that can be produced from lactose using cellobiose 2-epimerase and it considered developing prebiotic. In sense, the development of strategies to produce purify epilactose key for its wider use in food industry. The aim this work was establish food-grade purification strategy suitable scaled-up an industrial level. Firstly, by enzymatic epimerization reaction ca...

?-galactosidase is an enzyme capable of hydrolysing lactose, used in various branches industry, mainly the food industry. As efficient industrial use enzymes depends on their reuse, it necessary to find effective method for immobilisation, maintaining high activity and stability. The present work proposes cross-linked magnetic aggregates (mCLEAs) prepare heterogeneous biocatalysts ?-galactosida...

Lactose is the main carbohydrate in human and mammalian milk. Lactose requires enzymatic hydrolysis by lactase into D-glucose and D-galactose before it can be absorbed. Term infants express sufficient lactase to digest about one liter of breast milk daily. Physiological lactose malabsorption in infancy confers beneficial prebiotic effects, including the establishment of Bifidobacterium-rich fec...

β-galactosidase, commonly known as lactase, represents commercially important enzyme that is prevalently used for lactose hydrolysis in milk and whey. To the date, it has been isolated from various sources. In this study different strains of lactic acid bacteria were assessed for their β-galactosidase productivity, and Lactobacillus acidophilus ATCC 4356 resulted with the highest production pot...

β-galactosidase was successfully encapsulated within an amino-functionalised silica matrix using a "fish-in-net" approach and molecular imprinting technique followed by covalent binding of lysozyme via a glutaraldehyde-based method. Transmission electron microscopy (TEM), X-ray diffraction (XRD), scanning electron microscopy (SEM), and Fourier transform infrared (FTIR) spectroscopy were used to...

bGalactosidase (commonly known as b-lactase; EC 3.2.1.23) is a multifunctional enzyme that can catalyze the hydrolysis of terminal non-reducing b-D-galactose residues in b-D-galactosides or transfer the galactosyl residue to saccharide acceptors to yield galactooligosaccharides (GOS). b-Galactosidase has a variety of applications in food and medical industries such as hydrolysis of lactose in m...