The fermentation of lactose by salmonellae has seldom been accomplished, and in but few serological types (Kauffmann, 1954; Kristensen, 1948, 1955). In previous investigations we (Schafler and SchiLfler, 1959) succeeded in obtaining lactose-positive variants in 17 out of 30 salmonella strains belonging to different types, by means of 32 to 150 days of incubation on partially defined media conta...

Overall autophagy was measured in isolated hepatocytes as the sequestration and lysosomal hydrolysis of electroinjected [14C]lactose, using HPLC to separate the degradation product [14C]glucose from undegraded lactose. In addition, the sequestration step was measured separately as the transfer from cytosol to sedimentable cell structures of electroinjected [3H]raffinose or endogenous lactate de...

Lactose is the only soluble and economically feasible carbon source for the production of cellulases or heterologous proteins regulated by cellulase expression signals by Hypocrea jecorina (Trichoderma reesei). We investigated the role of the major beta-galactosidase of H. jecorina in lactose metabolism and cellulase induction. A genomic copy of the bga1 gene was cloned, and this copy encodes a...

and ADFI at week 4 (P = 0.02). During phase 2 (week 3 to 4), greater ADG (P = 0.05) and ADFI (P = 0.004) were observed in pigs consuming a diet containing DDGS (Treatments B and D) compared to pigs that did not receive DDGS in phase 1 (Treatments A and C); however, G:F ratio was not affected by DDGS. There was a lactose effect on ADG in week 3 (P = 0.05) and a trend of lactose effect on ADG dur...

The American Academy of Pediatrics Committee on Nutrition presents an updated review of lactose intolerance in infants, children, and adolescents. Differences between primary, secondary, congenital, and developmental lactase deficiency that may result in lactose intolerance are discussed. Children with suspected lactose intolerance can be assessed clinically by dietary lactose elimination or by...

The objective was to study the cause of variation in digestibility of mixed linked β(1-3;1-4)-D-glucan (β-glucan) in the small intestine of growing pigs. The β-glucan is an important cell wall [dietary fiber (DF)] component of the endosperm of barley (Hordeum vulgare) and oats (Avena sativa). The digestibility of β-glucan in the small intestine from both cereals is among the highest of all DF c...

BACKGROUND Lactose intolerance (LI) is a common medical problem with limited treatment options. The primary symptoms are abdominal pain, diarrhea, bloating, flatulence, and cramping. Limiting dairy foods to reduce symptoms contributes to low calcium intake and the risk for chronic disease. Adaptation of the colon bacteria to effectively metabolize lactose is a novel and potentially useful appro...

A mathematical model of the lactose (lac) operon was developed to study diauxic growth on glucose and lactose. The model includes catabolite repression, inducer exclusion, lactose hydrolysis to glucose and galactose, and synthesis and degradation of allolactose. Two models for catabolite repression were tested: (i) cyclic AMP (cAMP) synthesis inversely correlated with the external glucose conce...

Tauhe present work shows the relation between kefir fermentation ability and carbohydrate uptake rate. This was examined in a model system containing kefir co-culture and lactose in order to study fermentation induced by yeasts and bacteria at the same time. Lactose uptake rate was recorded by using (14)C-labelled lactose. The effect of lactose, cell concentration and pH on lactose fermentation...