A total of 232 isolates of gram-negative bacteria were recovered from mammals, reptiles, and birds housed at Asa Zoological Park, Hiroshima prefecture, Japan. Forty-nine isolates (21.1%) showed multidrug resistance phenotypes and harbored at least one antimicrobial resistance gene. PCR and DNA sequencing identified class 1 and class 2 integrons and many beta-lactamase-encoding genes, in additio...

Bacteria that simultaneously produce multiple extended-spectrum beta-lactamases are frequently isolated. We report an Enterobacter cloacae isolate, ES24, producing four different beta-lactamases (AmpC type beta-lactamase, TEM-1, SHV-7, and a novel extended-spectrum beta-lactamase, SHV-30). Direct sequencing of bla(SHV) gene products gave a "double peak" at position 703, suggesting the presence ...

An Ambler class A β-lactamase gene, bla(CIA-1), was cloned from the reference strain Chryseobacterium indologenes ATCC 29897 and expressed in Escherichia coli BL21. The bla(CIA-1) gene encodes a novel extended-spectrum β-lactamase (ESBL) that shared 68% and 60% identities with the CGA-1 and CME-1 β-lactamases, respectively. bla(CIA-1)-like genes were detected from clinical isolates. In addition...

background: acinetobacter baumannii, is an opportunistic pathogen and is responsible for numerous nosocomial infections. in recent years, this microorganism has been resistant to a wide range of antibiotics. one of the most important mechanisms of resistance in this microorganism is production of metallo-beta-lactamases (mbls). objectives: the aim of this study was to detect vim- and imp-type m...

The infections that causes by Pseudomonas aeruginosa is usually responsible for the nosocomial of United States. Occurs in each year approximality 51,000 cases P. , and persons most risk infection are those exposer into equipment hospital has not well desinfection such as ventilation mechanical catheters.Some strains mutate or production β-Lactamase enzymes resistance penicillins.The acts on di...

β-Lactamases are the main cause of bacterial resistance to penicillins, cephalosporins and related β-lactam compounds. These enzymes inactivate the antibiotics by hydrolysing the amide bond of the β-lactam ring. Class A β-lactamases are the most widespread enzymes and are responsible for numerous failures in the treatment of infectious diseases. The introduction of new β-lactam compounds, which...

BACKGROUND Gastroenteritis attributable to Salmonella enterica and the continuous increase in antimicrobial resistance of this gut pathogen, which compromises the use of previously effective treatments, is of great concern for public health. This study was conducted in order to investigate the presence of plasmid-mediated quinolone resistance (PMQR) determinants and β-lactamase-encoding genes, ...

background & objectives: pseudomonas aeruginosais one of the most important causes of nosocomial infections particularly in immunodeficient patients. several antibiotics such as betalactams, aminoglycosides and quinolones are used for the treatment of infections caused by this organism, but the prevalence of multidrug resistant strains has been reported frequently. beta lactamase production is ...