The interaction of L-aromatic aminoacids with bases and nucleosides is described. Of these, L-tryptophan interacts more strongly with bases and nucleosides as indicated by nuclear magnetic resonance, circular dichroism and solubility measurements. A possible ring-ring type interaction between the aminoacid and bases or nucleosides is proposed; this interaction being such that the hydrodinamic b...

In order to clarify the reaction mechanism of Mryptophan 2,3-dioxygenase (L-tryptophan: oxygen oxidoreductase, EC 1.13.1.12), a hemoprotein, spectral and kinetic studies were carried out with highly puritied enzyme preparations from Pseudomonas fluorescens (ATCC 11299). A new spectrally distinct species of the enzyme heme omx; 418,545, and 580 mp) was observed during the steady state of the cat...

The eosinophilia-myalgia syndrome (EMS) has been associated with ingestion of L-tryptophan (L-TRP) produced by a single manufacturer. Epidemiological data implicated 1,1'-ethylidenebis (L-tryptophan) (EBT) (peak 97 or peak E) as a possible etiologic agent. We showed previously that Lewis rats treated with the L-TRP implicated in EMS develop fasciitis and perimyositis similar to those seen in hu...

d-Tryptophan was converted to l-tryptophan in tissue cultures of tobacco, in whole cells treated with dimethylsulfoxide, and in cell-free extracts treated by Sephadex G-25 filtration. Evidence was obtained that tryptophanase, tryptophan pyrrolase, and transaminase activities were not involved. The data were best explained by the presence of a tryptophan racemase as the enzyme catalyzing the rea...

Calendula officinalis L. is primarily cultivated as cut flowers plant and also regarded a medicinal with variety of therapeutic uses. Irrigation management results in reduction irrigation requirements, water saving conservation. Tryptophan has major role improving stress tolerance to environmental stresses such deficiency. This study investigated the response C. under drip system number times w...

A 28-year-old female patient, housewife, presented to our outpatient department with a history of gradually increasing thickening and tightness of skin mainly over the extremities and face of three months duration. The patient had her first delivery five months earlier. There was no history of any significant co-morbidities or associated symptoms. Patient did not give a history of dysphagia, ex...

The metabolism of acetyltryptophan has been studied by various workers. Baldwin and Berg (1) and Rose (2) have reported that acetyl-ntryptophan will not maintain nitrogen balance in the adult human; however, the acetylated L isomer was equal to an equivalent amount of L-tryptophan in this respect. In contrast with these results, Albanese et al. (3) report that, with the exception of a 5 per cen...

Two Fab fragments of the monoclonal anti dinitrophenyl (DNP) spin-label antibody AN02 were prepared by recombination of specifically deuterated heavy and light chains. In the recombinant H(I)L(II) all the tyrosines and phenylalanines were perdeuterated as were the tryptophan residues of the heavy chain. In the recombinant H(II)L(I) all the tyrosines and phenylalanines were perdeuterated as were...

Tryptophan 5-hydroxylase was partially purified from rat small intestine and characterized. The enzyme activity was mainly localized in the distal one-fourth of the small intestine. The enzyme required Fe(2+), 2-amino-4-hydroxy-6,7-dimethyl-5,6,7,8-tetrahydropteridine and oxygen for full activity. The pH optimum of the reaction was 8.0. The hydroxylation rate of d-tryptophan by the enzyme was o...