Pepstatin has been shown to be an extremely potent inhibitor of human renin (Ki = 1.3 X lo-lo M). Kinetic analyses by several techniques show reversible interaction of pepstatin with renin and classical noncofipetitive inhibition. The high affinity of pepstatin for renin allows kinetic determination of the molarity of the enzyme in partially purified preparations. This method has been validated...

In this paper, we prove effective estimates for the number of exceptional values and the totally ramified value number for the Gauss map of pseudo-algebraic minimal surfaces in Euclidean four-space and give a kind of unicity theorem.

This paperdescribes awayof organizing information about an object-orientedprogram’s execution. The context is our system for visualizing that execution. The critical constraints are completeness, compactness, and efficient retrieval. We describe our design and how it meets these constraints.

In this note we study a minimization problem for a vector of measures subject to a prescribed interaction matrix in the presence of external potentials. The conductors are allowed to have zero distance from each other but the external potentials satisfy a growth condition near the common points. We then specialize the setting to a specific problem on the real line which arises in the study of c...

Glutathionylspermidine is an intermediate formed in the biosynthesis of trypanothione, an essential metabolite in defence against chemical and oxidative stress in the Kinetoplastida. The kinetic mechanism for glutathionylspermidine synthetase (EC 6.3.1.8) from Crithidia fasciculata (CfGspS) obeys a rapid equilibrium random ter-ter model with kinetic constants K(GSH) = 609 microM, K(Spd) = 157 m...

The successful manufacture of thick-sectioned composites is challenging, since the highly exothermic nature of thermoset resins and limited temperature control make it difficult to avoid detrimental thermal and cure gradients within the composite. In order to make quality parts, it has been found experimentally that cure temperatures must be lowered as much as 50% from those suggested for thin ...

Inhibition of lipoxygenase (LO) is currently an important goal of biomedical research due to its critical role in asthma, atherosclerosis, and cancer regulation. Steady-state kinetic data indicate that oleic acid (OA) is a simple competitive inhibitor for soybean lipoxygenase; however, kinetic isotope effect (KIE) data suggest a more complicated inhibitory mechanism. To investigate the inhibito...

The enzyme inosine monophosphate dehydrogenase (IMPDH) is responsible for the rate-limiting step in guanine nucleotide biosynthesis. Because it is up-regulated in rapidly proliferating cells, human type II IMPDH is actively targeted for immunosuppressive, anticancer, and antiviral chemotherapy. The enzyme employs a random-in ordered-out kinetic mechanism where substrate or cofactor can bind fir...

Red sea bream iridovirus (RSIV) has been identified as the causative agent of a serious disease in red sea bream and at least 30 other marine fish species. We developed a viral DNA microarray containing 92 putative open reading frames of RSIV to monitor the viral gene transcription program over the time course of an in vitro infection and to classify RSIV transcripts into temporal kinetic expre...