human alpha 1-antitrypsin (aat) cdna was obtained from hepg2 cell lines. after pcr and construction of expression vector ppiczα-aat, human aat was expressed in the yeast pichia pastoris (p.pastoris) in a secretary manner and under the control of inducible alcohol oxidase 1 (aox1) promoter. the amount of aat protein in medium was measured as 60 mg/l 72 hr after induction with methanol. results i...

background: the potential of plants as a safe and eukaryotic systems, are considered for producing recombinant therapeutic human protein today; but the expression level of heterologous proteins is limited by the post-transcriptional gene silencing (ptgs) response in this new technology. the use of viral suppressors of gene silencing can prevent ptgs and improve transient expression level of for...

in this study tsga10 has been demonstrated as a testis-specific human gene that encodes a protein localized in sperm-tail and conserved in ciliary structure. further investigations showed tsga10 signalling and expression during embryogenesis, brain development and some malignancies including brain tumors. given the role of this protein in neuronal development and in certain tumors, it could pot...

Introduction: Some strains of Escherichia Coli, including intestinal pathogenic strains, commensal strains, and extra intestinal pathogenic E. coli (ExPEC) have a significant impact on human health status. A standard vaccine designed based on conserved epitopes can stimulate a protective immune response against these pathogens. Additionally, enhanced expression at the infection site as a pathog...

Homeobox genes encode transcription factors which play important roles in the developmental processes of many multicellular organisms. TGIFLX/Y (TGIFLX and TGIFLY) are members of the homeobox superfamily of genes. Their expressions are specifically detected in the human adult testis but their functions are remained to be investigated. In this investigation we cloned full length of TGIFLY cDNA a...

Abstract Ethanol has a vast consumption around the world. Many researches confirmed some adverse effect of this component on human health. In addition, recent studies showed significant alteration in both cellular population, and protein profile of human foreskin fibroblast cell line (HFFF2) in the specific dosage of ethanol. Here, the role and interaction of some proteins (characterized by sig...

the oil and protein content of three varieties of water melon seeds namely, chillian black, dixy queen and charlston gray were determined. the soluble proteins were studied and measured by paper chromatography and amino acid analyzer. the results indicated that oil content of the seeds was 43, 53 and 45% and protein content was 27, 23, and 34% respectively. the soluble proteins contained all th...