نتایج جستجو برای: karim khans victories over his rivals

تعداد نتایج: 1287356  

Journal: :Biochimica et biophysica acta 2001
J G Altin F A White C J Easton

This work describes the synthesis and use of the chelator lipid, nitrilotriacetic acid ditetradecylamine (NTA-DTDA). This lipid is readily dispersed in aqueous media, both alone and when mixed with carrier lipids like dimyristoylphosphatidylcholine (DMPC). Fluorescence microscopic examination of membranes deposited from NTA-DTDA-containing liposomes shows that NTA-DTDA mixes uniformly with the ...

Journal: :Genome research 2006
Mohammad Arifuzzaman Maki Maeda Aya Itoh Kensaku Nishikata Chiharu Takita Rintaro Saito Takeshi Ara Kenji Nakahigashi Hsuan-Cheng Huang Aki Hirai Kohei Tsuzuki Seira Nakamura Mohammad Altaf-Ul-Amin Taku Oshima Tomoya Baba Natsuko Yamamoto Tomoyo Kawamura Tomoko Ioka-Nakamichi Masanari Kitagawa Masaru Tomita Shigehiko Kanaya Chieko Wada Hirotada Mori

Protein-protein interactions play key roles in protein function and the structural organization of a cell. A thorough description of these interactions should facilitate elucidation of cellular activities, targeted-drug design, and whole cell engineering. A large-scale comprehensive pull-down assay was performed using a His-tagged Escherichia coli ORF clone library. Of 4339 bait proteins tested...

Journal: :Protein science : a publication of the Protein Society 1998
A D Ferguson J Breed K Diederichs W Welte J W Coulton

FhuA (Mr 78,992, 714 amino acids), siderophore receptor for ferrichrome-iron in the outer membrane of Escherichia coli, was affinity tagged, rapidly purified, and crystallized. To obtain FhuA in quantities sufficient for crystallization, a hexahistidine tag was genetically inserted into the fhuA gene after amino acid 405, which resides in a known surface-exposed loop. Recombinant FhuA405.H6 was...

Journal: :Nucleic acids research 1994
M Ono P W Tucker J D Capra

Ku is an ubiquitous nuclear heterodimeric protein consisting of p70 and p86 subunits that binds double-stranded DNA termini and associates with chromosomes in vivo. It was originally described as an autoantigen in patients with certain autoimmune diseases. The individual subunits of Ku have been difficult to isolate from human cells without denaturation and attempts to produce functional recomb...

Journal: :Proceedings of the National Academy of Sciences of the United States of America 2000
Y C Lee E Martin F Murad

The alpha1- and beta1-subunits of human soluble guanylate cyclase (sGC) were coexpressed in the Sf9 cells/baculovirus system. In addition to the native enzyme, constructs with hexahistidine tag at the amino and carboxyl termini of each subunit were coexpressed. This permitted the rapid and efficient purification of active recombinant enzyme on a nickel-affinity column. The enzyme has one heme p...

پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه سمنان - دانشکده علوم انسانی 1393

colonialism is an important factor in cultural production and many of cultural constructs all over the world. english and english language teaching are at the center of the contemporary world. postcolonial literature, the genre achebe exercised, is a kind of historical text which has become central to different debates and criticisms. in this thesis, different elements related to the colonial...

Journal: :Archives of sexual behavior 2014
Katherine N Hanson Sobraske Steven J C Gaulin James S Boster

The costs imposed by a romantic partner's mixed reproductive strategy (MRS) generate selection pressures for anticipatory responses to mitigate or avoid those costs. People will differ in their vulnerability to those costs, based in part on the qualities of their romantic rivals. Thus, we predicted that individuals at high risk of a partner's MRS--women with many sexually accessible rivals and ...

Journal: :Mongolian Journal of International Affairs 2022

The developing field of Mongolian International Studies offers a diverse range research topics. A review recent articles reflects an emphasis on geo-politics, particularly evolving relations with its superpower neighbours. Whilst state-to-state engagement China and Russia predominates, regional countries (Japan, Korea) the US Europe are examined within ‘Third Neighbour’ policy. Trade economics ...

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