Antibiotic resistance is a major global health problem and continues to increase, so there need forbetter evaluation therapeutic advice for Urinary Tract Infection (UTI) patients. It had isolated E. colifrom clinical isolates that were resistant antibiotics of β-lactam, the first generation cephalosporinand fluoroquinolone. Characterization bacterial sensitivity should be analyzed other antibio...

Recently we reported on the rapid inactivation of tetracycline by use of cupric-morpholine complex (Kaplan, Lannon, and Buckwalter, J. Pharm. Sci., 64, 163, 1965). This degradation procedure is used as part of an assay technique for kanamycin in the presence of tetracycline. We have since found that sodium borohydride (Metal Hydrides Inc., Beverly, Mass.) can substitute for cupric-morpholine co...

An A1400G mutation of the rrs gene was identified in Mycobacterium tuberculosis (MTB) strain ATCC 35827 and in 13 MTB clinical isolates resistant to amikacin-kanamycin (MICs, >128 microg/ml). High-level cross-resistance may result from such a mutation since MTB has a single copy of the rrs gene. Another mechanism(s) may account for high-level amikacin-kanamycin resistance in two mutants and low...

introduction: the incidence of multidrug resistant tuberculosis is increasing in developing countries. aminoglycosides are an integral part of second-line drugs, however ototoxicity is a major limitation for their use. this study aims to determine the extent of hearing loss in patients taking one of the commonly prescribed drugs for multidrug resistant tuberculosis (mdr-tb), kanamycin, at a gov...

Aerosols of kanamycin resulted in greater survival of mice challenged with respiratory Klebsiella pneumoniae than the same dosage given intramuscularly. Determinations of viable bacteria in the blood and lungs revealed that aerosolized kanamycin was most effective when infection was confined to the lungs. After the organisms had spread to other areas, however, aerosol therapy was still more eff...

BB-K 8, a new semisynthetic aminoglycosidic antibiotic, was evaluated for ototoxicity, nephrotoxicity and neuromuscular blocking activity in the cat. As a reference, kanamycin was tested by the same experimental methods. Employing equivalent doses in terms of the free base and 7 days of intraperitoneal treatment, BB-K8 and kanamycin were found to be similarly ototoxic and nephrotoxic. Acutely, ...

Paromamine1>2) is a common and antibacterial moiety of paromomycin I and II, and kanamycin C, and was recently isolated3) from a crude kanamycin-complex. The synthesis4) had already been achieved in our laboratory through the condensation of bis-N,N/-(2,4-dinitrophenyl)-2-deoxystreptamine with 3,4,6-tri-O-acetyl-2-deoxy-2-(2,4dinitroanilino)-^-D-glucopyranosyl bromide. This paper describes an i...

Sir: As reported previously,1-5) aminoglycoside 6'-N-acetyltransferases [AAC(6')] in R factorcarrying Escherichia coli and Pseudomonas aeruginosa are involved in the mechanism of resistance to several aminoglycoside antibiotics such as kanamycin, kanamycin B, 3',4'dideoxykanamycin B (DKB) and ribostamycin. Gentamicin C1a and sisomicin are acetylated by these enzymes6,7). In this paper, we repor...

A chimeric octopine synthase-neomycin phosphotransferase (ocs-nptII) gene was used to transform Chlamydomonas reinhardiii to kanamycin resistance. Southern hybridization using DNA isolated from one transformant, T6.1, indicated that the entire ocs-nptII gene and at least part of the plasmid were integrated into nuclear DNA. Neomycin phosphotransferase II activity has been detected in T6.1 cell ...

Two novel systems were evaluated for performing indirect kanamycin susceptibility tests on 72 strains of Mycobacterium tuberculosis. The microplate Alamar blue colorimetric method (breakpoint, 2.5 microg/ml) and the Mycobacterium Growth Indicator Tube (MGIT) system (breakpoint, 5.0 microg/ml) both produced 98.6% agreement when compared with the conventional proportion method performed on 7H10 a...