An improved and reliable ultra-performance liquid chromatography/tandem mass spectrometry (UPLC-MS/MS) method has been developed and validated for the determination of lercanidipine in human plasma. Plasma samples with lercanidipine-d3 as an internal standard (IS) were prepared by solid phase extraction on Phenomenex Strata-X cartridges using 100 µL of human plasma. Chromatographic analysis was...

An automated solid phase extraction (SPE) protocol to determine carbamazepine in human serum has been developed and validated using a simple, rabid sensitive liquid chromatography-based bio-analytical method. Extraction of was carried out an on-line SPE tool short protein-coated (PC) ODS silica pre-column (PC-ODS-pre-column) phosphate buffer saline (PBS) with pH 7.4 as solvent. There are two di...

GERI-BP001 compounds, new inhibitors of acyl-CoA:cholesterol acyltransferase (ACAT), were isolated from a culture broth of Aspergillus fumigatus F37 by acetone extraction, EtOAc extraction, SiO2 column chromatography, and reverse phase HPLC. GERI-BP001 M, A, and B inhibit ACAT activity in an enzyme assay system using rat liver microsomes by 50% at concentrations of 42, 94, and 40 microM, respec...

The seed lipid of Nitraria tangutorum was extracted by supercritical carbon dioxide extraction, microwaveassisted reflux extraction, ultrasound-assisted extraction, or solvent reflux extraction. The experimental parameters of supercritical carbon dioxide extraction including pressure, temperature, particle size, and extraction time were investigated. A facile and sensitive method for the simult...

The aim of this study was to evaluate the repeatability and performance of 4 methods of extracting DNA from Staphylococcus aureus (SAU) and the gene encoding bovine mitochondrial cytochrome B (BMCB) in milk samples from cows with subclinical mastitis for use in amplification by real-time polymerase chain reaction. Two milk samples were obtained from cows naturally infected with S. aureus and su...

Solid-state extraction of plasma or serum offers significant advantages over traditional liquid-liquid methods in cortisol determinations by liquid chromatography (HPLC). Sample preparation is facilitated and HPLC column contamination is minimized. Extract yield from the solid-state columns is enhanced by sample pretreatment with methanol. Cortisol values by solid-state extraction/HPLC correlat...

The alternative fuel butanol can be produced via acetone-butanol-ethanol (ABE) fermentation from renewable resources, i.e. biomass. Expensive feedstocks and the high costs for the separation of ABE from the dilute fermentation broth in the downstream processing have so far prohibited the industrial-scale production of bio-butanol. The low productivities and butanol yields of ABE batch fermentat...

The NucliSENS easyMAG automated system was compared to the column-based Qiagen method for Epstein-Barr virus (EBV) or cytomegalovirus (CMV) DNA extraction from whole blood before viral load determination using the corresponding R-gene amplification kits. Both extraction techniques exhibited a total agreement of 81.3% for EBV and 87.2% for CMV.

For countries where catalytic cracking gasoline is the primary source, proposed technology consists in separating a benzene-rich fraction from order to be processed further together with reforming unit dedicated aromatics extraction. In this way, two benefits are obtained: as raw material for extraction and leftover that satisfies benzene content standards qualified product. It established use ...

A CMOS implementation of a one-dimensional Rtransform with 32 inputs is presented. The implementation generates a translation invariant feature vector from a set of spatially distributed analog inputs. Repeated application to the rows and columns of an image achieves two-dimensional translation invariance. Results are presented that demonstrate the translation invariance.