Trichoderma longibrachiatum IMI061758 possesses a powerful cellulose enzyme complex composed by different cellobiohydrolase, endoglucanase and /3-glucosidase activities. Recently we have cloned the T. longibrachiatum egll gene encoding a /S-{l,4)-endoglucanase activity (1). From an industrial point of view, we are interested in the design of strains which have an increased egll gene dosage. To ...

We evaluated the performance of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS), the MALDI Bruker Biotyper system (microflex LT; Bruker Daltonik GmbH, Bremen, Germany), on the identification of 50 isolates of clinically encountered molds, including Penicillium marneffei (n = 28), Paecilomyces species (n = 12), Fusarium solani (n = 6), Rhizopus species...

Para la caracterización microbiológica y molecular se aislaron e identificaron microorganismos cultivables de 4 bioinsumos comerciales provenientes del occidente norte Nicaragua. Esto involucró tipificación morfológica a través de: tinción Gram para bacterias observación esporas hongos filamentosos. Se realizó extracción secuenciación ADN (gen ADNr 16S – bacterias, región ITS1- ITS4 filamentoso...

High-throughput amplicon sequencing that primarily targets the 16S ribosomal DNA (rDNA) (for bacteria and archaea) Internal Transcribed Spacer rDNA fungi) have facilitated microbial community discovery across diverse environments. A three-step PCR utilizes flexible primer choices to construct library for Illumina has been applied several studies in forest agricultural systems. The protocol, whi...

Abstract. Hartanti AT, Susanti FN, Prasasty VD, Radiastuti N. 2021. Culturable endophytic fungal diversity in cassava tubers of Indonesia. Biodiversitas 22: 1250-1260. Fungal endophytes are found plant tissue and do not harm the host plant. Cassava (Manihot esculenta Crantz) is third staple food besides rice corn. This study aimed to identify fungi from several regions Indonesia, including Bogo...

A culture-independent mycobiome survey in Haplic Cambisol under Korean pine a long-term field experiment the Russian Far East was conducted using sequence analysis of ITS region amplified with ITS3/ITS4 primers metagenomic DNA as matrix. Overall 758 fungal OTUs were identified, representing 15 phyla, 47 classes, 104 orders, 183 families, and 258 genera. More represented Ascomycota phylum (513) ...

Abstract. Bahry MS, Radjasa OK, Trianto A. 2021. Potential of marine sponge-derived fungi in the aquaculture system. Biodiversitas 22: 2883-2892. Organic waste from is one triggers disease outbreaks and a decrease water quality that urgently needs to be resolved. Indonesia has high diversity sponges including their associated microorganisms potential field biotechnology. This study aimed determ...