The rational use of valuable fish species from aquaculture is difficult to implement without knowledge the state genetic structure local stocks. Different types DNA markers can be used achieve goals selection and breeding work. a stock rainbow trout (Oncorhynchus mykiss Walbaum, 1792) (Salmoniformes, Salmonidae) farmed in Ukraine was studied using DNA-markers: microsatellite (SSR-markers – simp...

Various methods have been used to identify cultivares of olive trees; herein we used different bioinformatics algorithms to propose new tools to classify 10 cultivares of olive based on RAPD and ISSR genetic markers datasets generated from PCR reactions. Five RAPD markers (OPA0a21, OPD16a, OP01a1, OPD16a1 and OPA0a8) and five ISSR markers (UBC841a4, UBC868a7, UBC841a14, U12BC807a and UBC810a13)...

In the present study, genetic fingerprints of ten species of Zingiberaceae from eastern India were developed using PCR-based markers. 19 RAPD (Rapid Amplified polymorphic DNA), 8 ISSR (Inter Simple Sequence Repeats) and 8 SSR (Simple Sequence Repeats) primers were used to elucidate genetic diversity important for utilization, management and conservation. These primers produced 789 loci, out of ...

DNA based molecular markers allow precise, objective and rapid cultivar identification. In this study, five ISSR and two sets of fluorescence-labeled TP-M13-SSR markers were applied for discrimination of eight closely related wheat cultivars. Five ISSR primers revealed a total of 43 distinct reproducible bands, 29 (67.44%) of the 43 bands were polymorphic. The number of polymorphic bands detect...

Aconitum is a medicinal treasure trove that grows extensively on fertile pastures in Xinjiang Province (China); however, its molecular genetic characteristics are still poorly studied. We studied Aconitum kusnezoffii Reichb., Aconitum soongaricum Stapf., Aconitum carmichaelii Debx. and Aconitum leucostomum Worosch, using random amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (...

Acrocephalidae is the most monomorphic family among passerines and has seen a long history of different classifications and successive revisions. In this study, we evaluated the phylogenetic relationships among 35 species of Acrocephalidae based on DNA sequences from five nuclear loci (MB, ODC, LDH, FIB5 and RAG-1), three mitochondrial genes (CYB, ND2 and COI) and genomic fingerprinting with IS...

For a study of diversity and genetic structuring in Melipona quadrifasciata, 61 colonies were collected in eight locations in the state of Minas Gerais, Brazil. By means of PCR analysis, 119 ISSR bands were obtained, 80 (68%) being polymorphic. H(e) and H (B) were 0.20 and 0.16, respectively. Two large groups were obtained by the UPGMA method, one formed by individuals from Januária, Urucuia, R...

Abstract Bitter gourd wilt caused by Fusarium oxysporum f. sp. momordicae (FOM) is a devastating crop disease in China. A total of 173 isolates characteristic typical with abundant microconidia and macroconidia on white or ruby colonies were obtained from diseased plant tissues. BLASTn analysis the rDNA-ITS showed 99% identity F. species. Among tested isolates, three infectious toward tower fiv...

The genus Tamarix consists of about 54 species that mainly grow in saline areas deserts and semi-deserts. This is chemically characterized by the presence tannins, flavonoids, anthocyanins essential oils which interfere with extraction pure genomic DNA. Thus it necessary to optimize protocols minimize influence these compounds lowest level. present study compares efficiency five different appro...

Pecan is an important horticultural nut crop originally from North America and now widely cultivated in China for its high ecological, ornamental and economic value. Currently, there are over one hundred cultivars grown in China, including introduced American cultivars and Chinese seedling breeding cultivars. Molecular markers were used to assess the genetic diversity of these cultivars and to ...