There is intense interest in determining the absolute abundance of specific proteins in complex mixtures, for example in the area of disease biomarker discovery. We have developed a set of protein-tagging reagents called visible isotope-coded affinity tags (VICAT reagents) that contain a protein-tagging reagent for reaction with cysteine SH groups, a visible probe for monitoring the chromatogra...

V a r i o u s imaging methods have been used for the preoperative localization of parathyroid tumors in patients with hyperparathyroidism. Among the noninvasive methods, several scintigraphic techniques have been employed, including variations on the dual-isotope 2°ITI/99mTC subtraction techniques (1-3). Modification of this technique, using 123I instead of [99mTC] pertechnetate, has also been ...

The enzyme-substrate complex is inherently transient, rendering its detection difficult. In our framework designed for bisubstrate systems-isotope-labeled, activity-based identification and tracking (IsoLAIT)-the common substrate, such as S-adenosyl-l-methionine (AdoMet) for methyltransferases, is replaced by an analogue (e.g., S-adenosyl-l-vinthionine) that, as a probe, creates a tightly bound...

Future missions to Venus will require a focused investigation of the atmospheric composition, to complete the story of its formation and evolution drafted by the probes of the 1970s and 1980s and developed further by recent missions such as Venus Express. Priority measurements include the mixing ratios and isotope ratios of all noble gases, detection of trace species in the lower atmosphere inc...

One of the main challenges facing experiments with rare-isotope beams is the need to extract high quality data of key physical observables from extremely limited numbers of incident ions and ever decreasing production cross sections for nuclei furthest from stability. Experiments are performed in inverse kinematics and must rely on thick reaction targets but this is often with the cost of a sig...

Stable isotopes (Deuterium and Oxygen-18) have a broad application in many of water related sciences. Precipitation is the most important input to the water cycle which shows considrable spatial and temporal variability in its isotope content. To study the precipitation isotope content, 51 samples from August 2015 to July 2016 have been collected mainly on a monthly basis with few events based ...

Benzoylecgonine (BEG) is the principal urinary metabolite of cocaine. For forensic drug testing, the presence of BEG in urine, suggested by a positive result for a screening immunoassay, must be confirmed by quantitative gas chromatographic/mass spectrometric (GC/MS) analysis, i.e., stable isotope dilution with a deuterium-labeled internal standard. GC/MS criteria for positivity also require ap...

Solid-phase DNase I footprinting provides a powerful tool for analyzing the sequence-specific interactions of DNA binding proteins. Classically this type of assay requires radioactively labeled DNA molecules. Substitution of the isotope by fluorescein labeling of the DNA fragments enables the analysis of footprint patterns on a standard automated laser fluorescent (A.L.F.) DNA Sequencer. The co...