Background and objectives: Tuberculosis (TB) is the major public health problem in the world. Each year there are 2-3 million deaths worldwide caused by TB. The increasing incidence of Multi Drug Resistance tuberculosis (MDR-TB) worldwide highlights the urgent need to search for new antituberculosis compounds. It has been reported that medicinal plant, Dracocephalum kotschyi, possesses some ant...

INTRODUCTION: This study aimed to evaluate a new commercial kit, Kit SIRE Nitratase-PlastLabor, for testing the drug susceptibility of clinical Mycobacterium tuberculosis isolates. METHODS: The accuracy of the Kit SIRE Nitratase was evaluated by examining the susceptibility (streptomycin, isoniazid, rifampicin, and ethambutol) of 40 M. tuberculosis isolates, using the proportion method with L...

sam (1956). Peizer and Widelock (1955), however, failed to find a consistent relationship between isoniazid sensitivity and catalase activity. Since the catalase test is easily performed and gives an immediate result, it would obviously be of value as a presumptive indicator of isoniazid resistance if it were proved to be consistently negative in isoniazid-resistant cultures. In order to obtain...

A whole genome sequencing was performed of strain M. tuberculosis 11502 (NCBI biosamples database, access code SAMN17832565) that assigned to the Beijing genotype subtype B0/W148 cluster 100-32, based on MIRU- VNTR loci (n = 24) structure, a nd t hat exhibited pre-extended d rug resistance. resistant isoniazid, rifampicin, ethambutol, levofloxacin, and ethionamide, which correlated with presenc...

OBJECTIVES The purpose of this study was to determine the levels of isoniazid and ethionamide resistance and to identify associated mutations in endemic multidrug-resistant (MDR) strains of Mycobacterium tuberculosis from the Lisbon metropolitan area, Portugal. METHODS Seventeen clinical MDR tuberculosis (TB) strains were characterized by standard and semi-quantitative drug susceptibility tes...

The continuing rise in tuberculosis incidence and the problem of drug resistance strains have prompted the research on new drug candidates and the mechanism of drug resistance. Molecular docking and molecular dynamics simulation (MD) were performed to study the binding of isoniazid onto the active site of Mycobacterium tuberculosis enoyl-acyl carrier protein reductase (InhA) in an attempt to ad...

Anti-tuberculosis drug resistance is a major problem in tuberculosis (TB) control, particularly multi-drug resistance TB (MDR-TB). The objective of this study was to determine the prevalence of primary and acquired anti-TB drug resistance among newly diagnosed pulmonary TB (PTB) and relapse cases. Sputa were collected from newly diagnosed and relapse PTB patients. Drug susceptibility tests (DST...

background : multi drug-resistant mycobacterium tuberculosis (mdr-tb) is an infection with a causative bacillus which is resistant to at least two drugs, isoniazid and rifampin. the purpose of this study is to evaluate the prevalence of tb resistance to first-line drugs of newly diagnosed active pulmonary tuberculosis. methods : this cross-sectional study was performed on 77 patients with newly...

this study was planned to determine hiv seroprevalence among pulmonary tuberculosis patients, to characterize the isolated mycobacteria into typical and atypical strains and to evaluate the drug resistant pattern of mycobacterial isolates. the study aims to correlate multidrug resistance (mdr) and hiv seropositivity status in pulmonary tuberculosis patients. during the year 1994-1997, 750 pulmo...