We have engineered a soluble, stable two-chain dimeric streptavidin (TCD) in Escherchia coli. Examination of the three-dimensional structure of streptavidin aided by empirical binding free-energy calculations helped us to select mutations at subunit interfaces that dissociate the native tetramer and stabilize the desired dimer. We chose positions W120, L124, V125 and H127 and mutated them to 12...

The water soluble A subunit of the human TSH receptor has been shown to have an isoelectric point of 5. As both TSH and TSH receptor antibodies have isoelectric points in the region of 8-10, charge-charge interactions must be of major importance in the binding of hormone or antibody to the TSH receptor A subunit.

1. The isolation of transferrin from rat serum by means of affinity chromatography on CNBr-activated Sepharose 4 B is described. -2. Subfractionation by isoelectric focusing yielded two transferrin fractions with identical biological behaviour but with small differences in isoelectric point (6.0 and 5.8) and sialic acid contents.

The isoelectric point of typhoid agglutinin lies between pH 4.4 and pH 4.6.

An extraordinarily large pi system based on tetrachloro-tetra(perylene bisimides) with very broad absorption spectra from the UV to the NIR region and an extremely strong electron-accepting ability has been synthesized from tetrachloro-di(perylene bisimides).

This article introduces a generalization of dimensional analysis and its corollary, the Pi-theorem, to the class of problems in which some of the quantities that define the problem have fixed values in all the cases that are of interest. The procedure can reduce the number of dimensionless similarity variables beyond the prediction of Buckingham's theorem. The generalized Pi-theorem tells when ...

Here we present the first empirical evidence to support the hypothesis that a gender-heterogeneous problem-solving team generally produced journal articles perceived to be higher quality by peers than a team comprised of highly-performing individuals of the same gender. Although women were historically underrepresented as principal investigators of working groups, their frequency as PIs at the ...