INTRODUCTION Resperidone chemically 4-[2-[4-(6-fluorobenzo [d] isoxazol-3-yl)-1-piper idyl] ethyl] -3methyl-2, 6diazabicyclo [4.4.0] deca-1, 3-dien-5-one. This drug belongs to a class of Anti-psychotic known as atypical neuroleptics. It is a strong dopamine antagonist. It has high affinity for D2 dopaminergic receptors. It has actions at several 5-HT (serotonin) receptor subtypes. The latter ac...

An isocratic sensitive and precise reverse phase high-performance liquid chromatography (RP-HPLC) method was developed and validated for the determination and quantification of hydrocortisone in controlled-release and conventional (tablets and injections) pharmaceutical preparations. Chromatographic separation was achieved on an ODS (C18), 5 μm, 4.6 × 150 mm, with an isocratic elution using a f...

The aim of pharmaceutical analysis is to obtain the necessary qualitative and quantitative information about the investigated sample. HPLC is the dominant separation technique in modern pharmaceutical and biomedical analysis because it results in highly efficient separations and in most cases provides high detection sensitivity. Chromatographic optimization procedures are becoming more multidis...

Two reversed-phase liquid chromatographic (RP-LC) methods are described for the determination of two binary mixtures of hypoglycemic agents. In the first method, vildagliptin (VDG) was determined in the presence of 3-amino-1-adamantanol (AAD), a synthetic intermediate and impurity of VDG. In the second method, pioglitazone hydrochloride (PGZ) and metformin hydrochloride (MET) were simultaneousl...

For selenium speciation analysis, the hyphenation of chromatographic separation with element-specific detection has proved a useful technique. A powerful separation system, which is capable of resolving several biologically and environmentally important selenium compounds in a single column, is greatly needed. However, that has been difficult to achieve. In this paper eight selenium compounds, ...

The objective of the current study was to develop and validate a simple, precise and accurate isocratic stability-indicating reversed-phase high-performance liquid chromatography (RP-HPLC) assay method for the determination of spironolactone and furosemide in solid pharmaceutical dosage forms. Isocratic RP-HPLC separation was achieved on an SGE 150 × 4.6 mm SS Wakosil II 5C8RS 5-μm column using...

A simple, specific and sensitive high-performance liquid chromatographic (HPLC) method for determination of S (-) Amlodipine Besylate (SAB) and its related substance i.e. R (+) Amlodipine Besylate (RAB) in tablet formulation was developed and validated. The isomer separation was carried out using simple isocratic reversed-phase HPLC method using a chiral column. Isocratic elution at a flow rate...

A reliable, rapid and sensitive isocratic reverse phase high-performance liquid chromatography method has been developed and validated for assay of ketorolac tromethamine in tablets and ophthalmic dosage forms using diclofenac sodium as an internal standard. An isocratic separation of ketorolac tromethamine was achieved on Oyster BDS (150×4.6 mm i.d., 5 μm particle size) column using mobile pha...

A simple, precise, and accurate, isocratic, reversed RP-HPLC stability indicating method was developed and validated for determination of Nabumetone (Naphthyl acetic acid derivative). Isocratic RP-HPLC separation was achieved on Inert Sil ODS 3V (5μ, 25 cm× 4.6 mm, i.d.) column using mobile phase consisted of 0.47 g of tetrabutyl ammonium hydroxide sulphate (TBAH) dissolved in 1000 ml of a mixt...