A reliable, rapid and sensitive isocratic reverse phase high-performance liquid chromatography method has been developed and validated for assay of ketorolac tromethamine in tablets and ophthalmic dosage forms using diclofenac sodium as an internal standard. An isocratic separation of ketorolac tromethamine was achieved on Oyster BDS (150×4.6 mm i.d., 5 μm particle size) column using mobile pha...

A simple, precise, and accurate, isocratic, reversed RP-HPLC stability indicating method was developed and validated for determination of Nabumetone (Naphthyl acetic acid derivative). Isocratic RP-HPLC separation was achieved on Inert Sil ODS 3V (5μ, 25 cm× 4.6 mm, i.d.) column using mobile phase consisted of 0.47 g of tetrabutyl ammonium hydroxide sulphate (TBAH) dissolved in 1000 ml of a mixt...

Simple, rapid and specific liquid chromatography-mass spectrometry (LC-MS) methods have been developed and validated for the quantification of cefcapene acid in human plasma and urine. Plasma samples were simply pretreated with methanol for deproteinization. Urine samples were briefly diluted with methanol-water (50:50, v/v), and centrifuged to remove large particles. Chromatographic separation...

Vitamin A (retinol), its metabolites and analogues have been heavily focused upon due to their clinical value and utility. Retinol can be used as a reflection of dietary or nutritional status, while the retinol analogues (isotretinoin, tretinoin, etretinate) are being proven as potent dermatologic and anti-tumor agents. High-performance liquid chromatography (HPLC) has been demonstrated to be a...

The main aim of present work was to develop a simple, precise, rapid and reproducible isocratic reverse phase high performance liquid chromatographic (RP-HPLC) method for the estimation of Imatinib in pure and in tablet dosage form. An isocratic RP-HPLC was performed by utilizing Welchrom C18 column, (250 mm × 4.6 mm i.d., particle size 5 μm) maintained at ambient temperature and mobile phase c...

The objective of the current study was to developed simple, precise and accurate isocratic reversedphase stability indicating HPLC assay method and validated for determination of ticlopidine hydrochloride in solid pharmaceutical dosage forms. Isocratic RP-HPLC separation was achieved on a Phenomenex Luna C8 (2) column (250 mm ́ 4.6 mm i.d., 5 μm particle size) using mobile phase of methanolammon...

A new mathematical treatment concerning the gradient elution in reversed-phase liquid chromatography when the volume fraction psi of an organic modifier in the water-organic mobile phase varies linearly with time is presented. The experimental ln k versus psi curve, where k is the retention factor under isocratic conditions in a binary mobile phase, is subdivided into a finite number of linear ...

Fluconazole(FLK) is a synthetic triazole derivative antifungal agent that has been shown to be effective against a wide range of systemic and superficial fungal infections. A simple, cost effective, precise, accurate, and stabilityindicating isocratic Ultra Performance Liquid Chromatographic (UPLC) method was developed and validated for the determination of FLK in bulk drug and in its tablets. ...

A reliable and sensitive isocratic stability indicating RP-HPLC method has been developed and validated for assay of rosuvastatin calcium in tablets and for determination of content uniformity. An isocratic separation of rosuvastatin calcium was achieved on YMC C8, 150×4.6 mm i.d., 5 μm particle size columns with a flow rate of 1.5 ml/min and using a photodiode array detector to monitor the elu...