Methods for separation of ions by ion-exchange, ion-pair, and zwitterion ion chromatography share at least one common thread--the induced formation of a cation-anion pair in the stationary phase. Selectivity can be defined as the relative ability of sample ions to form such a pair. Examples are given in anion-exchange chromatography to show the effect of variations in the geometry, bulkiness an...

introduction: polyclonal antibodies are required to be affinity purified. improved purification methods of polyclonal antibody provide an opportunity to pick the most purified immunoglobulins as a primary or secondary antibody in immunoassays that are included in many vaccine studies. two common techniques for purifying proteins is salt precipitation and chromatography purification. our work fo...

In this work, several machine learning techniques are presented for nanofiltration modeling. According to the results, specific errors are defined. The rejection due to Nanofiltration increases with pressure but decreases with increasing the concentration of chloride ion. Methods of machine learning represent the rejection of nanofiltration as a function of concentration, pH, pressure and also ...

Least-squares fitting of overlapping peaks is often needed to separately quantify ions in high-resolution mass spectrometer data. A statistical simulation approach is used to assess the statistical precision of the retrieved peak intensities. The sensitivity of the fitted peak intensities to statistical noise due to ion counting is probed for synthetic data systems consisting of two overlapping...

The effect of acetate ion introduced to aqueous solutions was investigated for the extraction/separation of Ni and Co from a dilute sulfate media. The separation of Co and Ni was improved by introducing acetate to aqueous media. By the use of the slope analysis method,the stoichiometric coefficient of the extractant was found to be 4 for cobalt and 3.5 or 4 for nickel depending on the aceta...

The fields of metabolomics and metabonomics attempt to phenotype and quantify the vast array of metabolites present in biological samples. Reversed-phase liquid chromatography (LC) coupled with mass spectrometry (MS) is a valuable tool in the separation and identification of these compounds. Reversed-phase HPLC techniques cover a wide range of compounds. However, ionic and polar compounds such ...