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Adsorption chromatography depends upon interactions of different types between solute molecules and ligands immobilized on a chromatography matrix. The first type of interaction to be successfully employed for the separation of macromolecules was that between charged solute molecules and oppositely charged moieties covalently linked to a chromatography matrix. The technique of ion exchange chro...

High nitrate concentrations in salads and vegetables have attracted increased attention. It is well know that nitrate can cause serious health problems to humans such as methemoglobinemia of infants 111. Under certain conditions, specially in the human body carcinogenic nitrosamines can be formed [2]. However, nitrate content of different types of vegetables fluctuates substantially with variet...

Enriched stable isotopes of many elements have been widely used in many aspects and such isotopes have been primarily used as the tracer for agricultural and biochemical studies and the availability of the isotopically labeled compounds have been now extensively increasing. Chemical exchange separation of isotopes is based on the equilibrium fractionation of isotopes between two phases, ion exc...

Despite numerous studies, isolating pure preparations of extracellular vesicles (EVs) has proven challenging. Here, we compared ion-exchange chromatography (IEC) to the widely used sucrose density gradient (SDG) centrifugation method for the purification of EVs. EVs in bulk were isolated from pooled normal human amniotic fluid (AF) by differential centrifugation followed by IEC or sucrose densi...

In Part 1 of this series of papers (Partridge & Westall, 1949) it was shown that, whilst the baseexchange resin Zeo Karb 215 may be used with success for the separation of the neutral and acidic amino-acids, certain difficulties arise when the separation of basic substances is attempted. These difficulties were traced to the phenolic hydroxyl groups of the resin. Resins of the sulphonated pheno...

Department of Analytical and Environmental Chemistry, Vilnius University, Naugarduko 24, LT-03225 Vilnius, Lithuania A simple and rapid ion pair chromatographic method has been developed for the determination of thiocyanate. Separation was carried out on a 25 cm × 4.6 mm Ascentis C18 column (5 μm particle size) with a mobile phase containing 2 mmol/l tetrabutylammonium (TBA)-phosphate (pH 6.5) ...