Invertases (P-fructofuranosidase, EC 3.2.1.26) have been studied in many organisms, including plants. At least two forms of the enzyme, soluble and particulate, are a common feature to a11 organisms. The soluble form is predominantly localized to vacuoles and cytoplasm and the particulate form, in a11 cases tested, is ionically bound to the cell wall (hence, cell-wall-bound form) and is readily...

Slowing down cold-induced sweetening (CIS) of potato (Solanum tuberosum) tubers is of economic importance for the potato industry to ensure high-quality products. The conversion of sucrose to reducing sugars by the acid invertase StvacINV1 is thought to be critical for CIS. Identification of the specific StvacINV1 inhibitor StInvInh2B and the α- and β-subunits of the interacting protein SUCROSE...

Thermotoga maritima invertase (beta-fructosidase), a member of the glycoside hydrolase family GH-32, readily releases beta-D-fructose from sucrose, raffinose and fructan polymers such as inulin. These carbohydrates represent major carbon and energy sources for prokaryotes and eukaryotes. The invertase cleaves beta-fructopyranosidic linkages by a double-displacement mechanism, which involves a n...

Saccharomyces cerevisiae is known to produce invertase. However, molecular characterization of successive yeast strains and their optimal invertase producing conditions in Nigerian Raphiahookeri has not been documented. In this study, fresh palm wine (Raphiahookeri) obtained from Lagos, Nigeria was evaluated. To achieve this, sequencing 18S rDNA carried out following DNA extraction the isolates...

Invertase is a commercially important enzyme used for the hydrolysis of sucrose. The hydrolysis of sucrose yields an equimolar mixture of glucose and fructose, known as invert syrup, is widely used in food and beverage industries. This enzyme is also used for the manufacture of artificial honey, plasticizing agents used in cosmetics, pharmaceutical and paper industries as well as enzyme electro...

To further understand how membrane proteins are sorted in the secretory system, we devised a strategy that involves the expression of a membrane-anchored yeast invertase in transgenic plants. The construct consisted of a signal peptide followed by the coding region of yeast invertase and the transmembrane domain and cytoplasmic tail of calnexin. The substitution of a lysine near the C terminus ...

Water deficit during male meiosis in wheat (Triticum aestivum L.) causes pollen sterility. With a view to identifying the internal trigger for this failure, it was found that water stress specifically impairs the activities of vacuolar and cell-wall invertases in anthers prior to the arrest of pollen development. The enzymes are affected only when water deficit occurs around meiosis. Three inve...

A short-term water deficit (WD) imposed during the pre-storage phase of lupin seed development [15-22 d after anthesis (DAA)] accelerated seed maturation and led to smaller and lighter seeds. During seed development, neutral invertase (EC 3.2.1.26) and sucrose synthase (EC 2.4.1.13) have a central role in carbohydrate metabolism. Neutral invertase is predominant during early seed development (u...

Mutants of Saccharomyces cereoisiae with defects in sucrose or raffinose fermentation were isolated. In addition to mutations in the SUCZ structural gene for invertase, we recovered 18 recessive mutations that affected the regulation of invertase synthesis by glucose repression. These mutations included five new snfl (Sucrose ponfermenting) alleles and also defined five new complementation grou...

Activities of the sucrose-cleaving enzymes, acid and neutral invertase and sucrose synthase, were measured in pods and seeds of developing snap bean (Phaseolus vulguris L.) fruits, and compared with t4C-import, elongation and dry weight accumulation. During the first 10d postanthesis, pods elongated rapidly with pod dry weight increase lagging behind by several days. The temporal patterns of ac...