In Coffea arabica (arabica coffee), the phenotypic as well as genetic variability has been found low because of the narrow genetic basis and self fertile nature of the species. Because of high similarity in phenotypic appearance among the majority of arabica collections, selection of parental lines for inter-varietals hybridization and identification of resultant hybrids at an early stage of pl...

A single-strand-specific nuclease from rye germ (Rn nuclease I) was characterized as a tool for secondary and tertiary structure investigation of RNAs. To test the procedure, yeast tRNA(Phe) and tRNA(Asp) for which the tertiary structures are known, as well as the 3'-half of tRNA(Asp) were used as substrates. In tRNA(Phe) the nuclease introduced main primary cuts at positions U33 and A35 of the...

abstract- a real-time, site-specific, machine-vision based, inter-row patch herbicide application system was developed and evaluated. the image resolution was 640 × 480 pixels covering a total area of 350 mm x 240 mm of a field composed of four quadrants of 350 mm x 60 mm each. the image frames were processed by labview® and matlab®. the developed algorithm, based on weed coverage ratio and seg...

We investigated the allele- and strand-specific transcriptional landscape of a megabase-wide genomic region of mouse Ube3a (ubiquitin protein ligase E3A) by means of a highly parallel SNP genotyping platform. We have successfully identified maternal-specific expression of Ube3a and its antisense counterpart (Ube3a-ATS) in brain, but not in liver. Because of the use of inter-subspecies hybrid mi...

Nowadays, information plays an important role in organizations. Sensitive information is often stored in databases. Traditional mechanisms such as encryption, access control, and authentication cannot provide a high level of confidence. Therefore, the existence of Intrusion Detection Systems in databases is necessary. In this paper, we propose an intrusion detection system for detecting attacks...

The origin of new diploid species through inter-specific hybridization may be facilitated by rapid genomic reorganization. There is evidence that this process was involved in the independent origins of three annual sunflower species in the genus Helianthus. The three hybrid taxa, H. anomalus, H. deserticola and H. paradoxus, are products of ancient hybridization events between the same two pare...

Experiments with somatic cell nuclear transfer, inter-cellular hybrid formation_ENREF_3, and ectopic expression of transcription factors have clearly demonstrated that cell fate can be dramatically altered by changing the epigenetic state of cell nuclei. Here we demonstrate, using chemical fusion, direct reprogramming of the genome of human embryonic fibroblasts (HEF) into the state of human fe...